重组人肝再生增强因子对5/6肾切除大鼠肾组织MMP-9、TIMP-1及Collgen-Ⅳ表达的影响  

Effects of rhALR on renal expressions of Collgen-Ⅳ,MMP-9 and TIMP-1 in rats after 5/6 renal nephrectomy

作　　者：迟雁青[1] 王学敏[2] 郭珊珊[1] 林海英[1] 张涛[1] 王秀芬[1] 丁芳[1] 刘茂东[1] 李英[1] 

机构地区：[1]河北医科大学第三医院,石家庄050051 [2]河北省冀州市医院

出　　处：《山东医药》2012年第8期55-56,59,共3页Shandong Medical Journal

基　　金：河北省科技厅科技支撑计划(10276105D-55)

摘　　要：目的探讨重组人肝再生增强因子(rhALR)是否可以通过影响基质金属蛋白酶9(MMP-9)、基质金属蛋白酶组织抑制因子1(TIMP-1)及Ⅳ型胶原(Collgen-Ⅳ)的表达,来延缓肾小球硬化。方法雄性SD大鼠分为假手术组、对照组及rhALR组,以rhALR对5/6肾切除所致慢性肾衰竭大鼠进行干预。结果与假手术组比较,对照组肾小球硬化指数增大,Collgen-Ⅳ、TIMP-1表达增加,MMP-9表达减少(P均<0.05);外源性给予rhALR能降低TIMP-1表达,增加MMP-9表达,减轻Collgen-Ⅳ的沉积,改善肾小球硬化(P均<0.05)。结论 rhALR可通过上调MMP-9/TIMP-1比例减少Collgen-Ⅳ积聚而改善肾小球硬化,保护残肾功能。Objective To investigate the effects of rhALR on MMP-9,Collgen-Ⅳ and TIMP-1 with 5/6 nephrectomy rats.Methods Sixty healthy male SD rats were randomly divided into three groups： sham operation group（SO,n=20）,CRF model group without treatment（C,n=20） and CRF model group treated with rhALR（rhALR,n=20）.Results Compared with group SO,the group C had a significantly higher glomerular sclerosis index and high level expression of Collgen-Ⅳ and TIMP-1,while the expression of MMP-9 was markedly decreased（P0.05）.The group dealt with rhALR had a down-regulating of expression of TIMP-1 and up-regulating of expression of MMP-9 compared with group C（P0.05）.Conclusions The rhALR acts as a factor contributing to decrease accumulation of Collgen-Ⅳ as well as protect the kidney against glomerular sclerosis by the up-regulating of expression of MMP-9 and the down-regulating of expression of TIMP-1 in renal tissues.

关 键 词：重组人肝再生增强因子 基质金属蛋白酶-9 金属蛋白酶组织抑制因子-1 Ⅳ型胶原 肾小球硬化 

分 类 号：R318.04[医药卫生—生物医学工程] R33[医药卫生—基础医学]