流式细胞术在克氏原螯虾血细胞的分类、活性和免疫功能研究中的应用  

Application of flow cytometry in studies of classification,viability andimmune parameters of Procambarus clarkii Haemocytes

作　　者：冼健安[1] 王安利[1] 苗玉涛[1] 

机构地区：[1]华南师范大学生命科学学院,广东省水产健康安全养殖重点实验室,高等学校生态与环境科学广东省重点实验室,广州510631

出　　处：《淡水渔业》2012年第1期9-14,57,共7页Freshwater Fisheries

基　　金：国家自然科学基金资助项目(30671628);广东省教育部产学研结合资助项目(2006D90204005);国家科技支撑计划项目(2007BAD29B06);广东省科技计划项目(2007B020708013);广东省重点科技项目(2009A020101006);广东省自然科学基金项目(5005909和021098);广州市科技支撑计划项目(2009Z1-E681)

摘　　要：应用流式细胞术(FCM)对克氏原螯虾(Procambarus clarkii)血细胞的分类、活性和免疫功能进行了研究。结果显示:血细胞可分为透明细胞、小颗粒细胞和大颗粒细胞三个亚群,组成比例分别为(26.25±5.29)%、(51.44±7.02)%和(11.20±1.82)%;螯虾血细胞的平均总凋亡率约为3.12%;血细胞对荧光大肠杆菌的吞噬活力显著(P<0.05)高于荧光微球,吞噬率分别为17.04%和14.57%;血细胞在自然生理状态下含有一定量的活性氧,其在两类颗粒细胞的含量显著(P<0.05)高于透明细胞,在大颗粒细胞中最高。结果表明,FCM能较好地应用于虾类的血细胞分类和功能研究。Classification, viabihty and immune function of crayfish （Procambarus clarkii） haemocytes were. studied using flow cytometry. Results showed that haemocytes were divided into three subpopulations, including hyaline cells, semigran- ular cells and granular cells, with the proportions of （ 26. 25 ± 5. 29 ） % , （ 51.44 ± 7.02 ） % and （ 11.20 ± 1.82 ） %, re- spectively. The average apoptotic ratio was 3.12% in the total haemocytes. Phagocytic activity against fluorescent E. coli was significantly higher than that of fluorescent microsphere （ P 〈 0. 05 ）. The phagocytic ratios were 17.04 and 14.57%, re- spectively. Haemocytes also produced reactive oxygen species at the natural physiological state. The reactive oxygen produc- tion was significantly higher in the two granular cells than hyaline cells （ P 〈 0. 05 ）, and highest in granular cells. The present study showed the superiority of FCM on study of cellular classification and functions of shrimp haemocytes.

关 键 词：流式细胞术 克氏原螯虾(Procambarus clarkii) 血细胞 

分 类 号：S917[农业科学—水产科学]