氮磷浓度对雨生红球藻叶绿素荧光参数的影响  

Effects of different nitrogen and phosphorus concentrations on the chlorophyll fluorescence parameters of Haematococcus pluvialis

作　　者：陈书秀[1,2] 梁英[1] 王虎 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003 [2]山东东方海洋科技股份有限公司,山东烟台264003 [3]烟台海融生物技术有限公司,山东烟台265600

出　　处：《淡水渔业》2012年第1期15-19,共5页Freshwater Fisheries

基　　金："十一五"国家科技支撑计划"海洋微藻高效培养工程化技术集成示范"(2006BAD09A03)

摘　　要：在一次性培养过程中,利用水样叶绿素荧光仪(Water-PAM)研究了2个单因子(不同氮浓度、不同磷浓度)对雨生红球藻(Haematococcus pluvialis)叶绿素荧光参数(PSⅡ的最大光能转化效率Fv/Fm、光合电子传递效率ETR)、叶绿素相对含量及细胞密度的影响。单因子方差分析结果表明,氮磷浓度对雨生红球藻的叶绿素荧光参数及生长均有显著的影响(P<0.05)。多重比较结果表明,雨生红球藻进行光合作用和生长最适氮浓度为1760μmol/L;最适磷浓度为18.16μmol/L。相关性分析结果表明:在整个培养周期中,叶绿素相对含量均与细胞密度呈显著的正相关关系。The effects of different nutrient concentrations （ nitrogen and phosphorus concentrations） on the maximal photo- chemical efficiency of PSII （ Fv/ Fm） , electron transport rate （ETR）, relative chlorophyll content and cell densities of Haematococcus pluvialis were studied by Water-PAM in batch cultures. One-way analysis of variance showed that N concen- trations and P concentrations had significant effects on the chlorophyll fluorescence parameters and growth of H. pluvialis. Multiple comparison tests showed that the optimal N concentrations was 1760 i.tM and the optimal P concentration was 18. 16 uLM for the photosynthesis and growth. The analysis of correlations showed that there were significant positive correla- tions between relative chlorophyll content and cell density during the entire experimental period.

关 键 词：雨生红球藻(Haematococcus pluvialis) 叶绿素荧光参数 氮浓度 磷浓度 生长 

分 类 号：S917.3[农业科学—水产科学] Q945.78[生物学—植物学]