牛支原体生长曲线的测定和培养基筛选  

Medium Screening and Determination of the Growth Curve of Mycoplasma bovis

作　　者：邵倩[1,2] 储岳峰[2] 何生虎[1] 逯忠新[2] 

机构地区：[1]宁夏大学农学院,银川750021 [2]中国农业科学院兰州兽医研究所/家畜疫病病原生物学国家重点实验室/农业部草食动物(疫病重点开放实验室/农业部畜禽病毒学重点开放实验室,兰州730046

出　　处：《湖北农业科学》2012年第3期549-551,共3页Hubei Agricultural Sciences

基　　金：甘肃省农业生物技术专项(GNSW-2010-09);甘肃省科技支撑计划项目(1011NKCA054);国家科技基础性工作专项计划(2008FY210200)

摘　　要：为筛选最适于牛支原体(Mycoplasma bovis)生长的培养基,利用活菌计数方法测定了牛支原体OF2株在改良Thiaucourt’s培养基、牛心汤培养基和改良KM2培养基中的生长曲线。结果发现,牛支原体在改良Thiaucourt’s培养基中生长30 h即可达到平台期,平均最高生长滴度7.0×109ccu/mL;在牛心汤培养基中生长40 h即可达到平台期,平均最高生长滴度4.0×109ccu/mL;在改良KM2培养基中生长50 h达到平台期,其平均最高生长滴度为7.0×108ccu/mL。结果表明,牛支原体在改良Thiaucourt’s培养基中生长快、滴度高,为下一步牛支原体灭活疫苗的研究提供了参考依据。To screen the suitable culture medium for Mycoplasma bovis,the growth curves of M.bovis strain OF2 in the inproved Thiaucourt＇s medium,the cattle-heart fusion medium and the inproved KM2 medium were determined by viable count method,respectively.The results showed that the stationary phase of M.bovis appeared after cultrured 30 h in the inproved Thiaucourt＇s medium,40 h in the cattle-heart fusion medium and 50 h in the inproved KM2 medium.The average highest growth titers of the OF2 strain were 7.0×109 ccu /mL,4.0×109 ccu /mL and 7.0×108 ccu /mL,respectively.It indicated that the growth rate and titers of M.bovis strain OF2 in the Thiaucourt＇s medium were the highest in the three selected media.It provided a useful data for the development of inactivated vaccine against M.bovis.

关 键 词：牛支原体(Mycoplasma bovis) 培养基筛选 生长曲线 

分 类 号：S852.62[农业科学—基础兽医学] S858.23[农业科学—兽医学]