用于Real-time PCR检测的牛肉组织DNA提取方法探索  

Exploration of Extraction Method of Beef Tissue's DNA for Real-time PCR Detection

作　　者：李璟[1] 陈世界 陈洋[1] 

机构地区：[1]成都理工大学材料与化学化工学院绿色催化合成研究所,成都610059 [2]四川省进出口检验检疫局技术中心,成都610041

出　　处：《湖北农业科学》2012年第5期1028-1029,1056,共3页Hubei Agricultural Sciences

基　　金：国家质检公益性项目(200910188)

摘　　要：使用Chelex-100提取牛肉组织DNA,并用紫外分光光度法检测所提取的DNA浓度和纯度,结果表明所提取的DNA A260 nm/A280 nm在1.662～1.826之间,纯度较高。Real-time PCR扩增提取的DNA模板,表现出较高的敏感性。该DNA提取方法效率较高,操作简便,能够获得较高质量的DNA,适合检疫部门的快速检测需要。Chelex-100 was used to extract DNA from beef tissue. The DNA samples were then tested by UV spectrophotome- try. The resnhs revealed that A260nm/A280nm, of DNA was 1.662-1.826. Real-time PCR results showed good sensitivity. The pro- cesses of DNA extraction method was simple, rapid and able to obtain DNA with high quality, which could be used for rapid detection of animal tissue by inspect and quarantine department.

关 键 词：DNA 提取 REAL-TIME PCR 牛肉组织 

分 类 号：Q953[生物学—动物学]