HPLC法测定大青叶中靛玉红的含量  

Measurement of Indirubin Content in Daqingye by HPLC

作　　者：王虹霞[1] 梁剑平[2] 李雪虎[3] 陆锡宏[3] 雷宏东[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050 [3]中国科学院近代物理研究所,甘肃兰州730000

出　　处：《湖南农业科学》2012年第2期87-89,共3页Hunan Agricultural Sciences

基　　金：甘肃省重大科技专项(092G K D A0033);国家青年基金项目

摘　　要：以HPLC法作为大青叶中靛玉红含量的检测方法,探讨了不同pH值和不同萃取溶剂对靛玉红提取的影响,确立了大青叶中靛玉红含量的测定方法。结果表明:在试验条件下,靛玉红在1.5～24μg/mL范围内与峰面积呈良好的线性关系(r=0.999 9),精密度RSD=0.64%,加样回收率为97.33%,重现性RSD=0.54%。HPLC法简单可行,准确性好,重现性高,是大青叶中靛玉红含量测定的有效方法。To establish a method for measuring the content of indirubin in Daqingye, the HPLC method was adopted to discuss the impacts of different pH values and different extraction solvents on extraction of indirubin from Daqingye. The results indicated that under the experimental conditions, indirubin showed a good linear correlation （r=0.999 9） with peak area within the range of 1.5 -24μg/mL, precision RSD =0.64%, the average recovery ratio was 97.33%, and the repeatability RSD=0.54%. Therefore, the HPLC method is a simple and feasible method with high accuracy and good reproducibility, which is an effective method for measurement Of indirubin content in Daqingye.

关 键 词：大青叶 靛玉红 HPLC 

分 类 号：S567[农业科学—中草药栽培]