青海湖嗜盐微生物筛选分离与生长特性  

Isolation and characteristics of halophile microorganisms from Lake Qinghai

作　　者：沈国平[1] 朱德锐[1] 刘建[2] 韩睿[3] 龙启福[1] 

机构地区：[1]青海大学医学院,西宁810016 [2]华中师范大学生命科学学院,武汉430079 [3]青海大学农林科学院,西宁810016

出　　处：《湖泊科学》2012年第2期313-319,共7页Journal of Lake Sciences

基　　金：国家自然科学基金项目(31060013)资助

摘　　要：为全面获得青海湖嗜盐菌种质资源,构建种群系统发育树和确定种群进化定位.采用高盐选择性培养基筛选,分离从20份青海湖水样中获得35株青海湖嗜盐微生物.耐盐梯度实验表明:水体中以中度嗜盐菌为主,约占62.85%,轻度嗜盐菌约占22.85%,非嗜盐菌与耐盐菌约占14.28%.青海湖中度嗜盐微生物能在10～45℃,pH 5.5～11.0的范围内生长,与地区盐碱化环境相关,具有嗜盐兼嗜碱微生物的特性.The main objective of the project is to obtain completely halophile germplasm resources from Lake Qinghai,to construct stocks phyletic evolution tree of halophilic bacterium and determine the evolution position.35 strains were isolated from 20 water samples taken from Lake Qinghai using the high salty medium.Resistant to salt gradient experiment shows that moderate halophilic bacteria account for about 62.85% in the water,mild halophilic bacteria account for about 22.85%,non halophilic bacteria and salt resistance bacteria account for 14.28%.Moderate halophilic bacteria could grow in the temperature of 10～45℃ and pH 5.5～11.0,which is related to the local salinization environment and has characteristic of hasophilic microorganisms.

关 键 词：青海湖 嗜盐菌 分离筛选 生长特性 

分 类 号：Q938.8[生物学—微生物学]