提高小麦×玉米中小麦单倍体苗加倍率的研究  被引量:8

Study on Improving Frequency of Chromosome Doubling in Wheat Haploid Produced by Wheat×Maize System

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作  者:顾坚[1] 杨木军[1] 李绍祥[1] 田玉仙[1] 李宏生[1] 杨和仙[1] 刘琨[1] 赵红[1] 

机构地区:[1]云南省农业科学院粮食作物研究所,云南昆明650205

出  处:《西南农业学报》2012年第1期19-21,共3页Southwest China Journal of Agricultural Sciences

基  金:云南省重点产业科技创新工程(2010BB0005);国家高技术研究发展计划(863计划)(2009AA101102)

摘  要:以小麦玉米杂交产生的小麦单倍体为材料,研究了单倍体苗分蘖节切口预处理对加倍率的影响。结果表明,加倍前对分蘖节进行切口处理的单倍体苗加倍率和加倍效率均达90.3%,比对照高16个百分点。对560个加倍成功的DH株的第一结实穗位分布进行了统计,从主茎开始结实的植株只占31.1%,其余均从分蘖穗开始结实,个别植株到第13个分蘖穗才收到种子;前5个穗位开始结实的植株占82.1%,前7个穗位占94.3%。结果表明,小麦单倍体的加倍率不仅受加倍方法的影响,还与加倍处理前后的栽培技术有关,培育多蘖壮苗、提高分蘖成穗率是提高加倍率的重要保障。Effects of incising the tillering node of haploid seedling before treated with colchicine on the frequency of chromosome doubling were investigated.Results indicated that the pre-treatment of incising tillering node obviously increased the doubling frequency from 74.3 %(CK) to 90.3 %. According to the analysis of ear order beginning seed-set in 560 doubled haploids,only 31.1 % doubled plants begin seed-set from the main stem,most from the tiller ears,varying from the first to the thirteenth tiller,the total proportion of seeding from the top five ears were 82.1 %,that from the top seven ears were 94.3 %,suggesting that developing as many tillers and tiller ears as possible for haploid through cultivation before and after doubling was the guarantee of getting higher doubling frequency.

关 键 词:小麦玉米杂交 单倍体 加倍率 

分 类 号:S512.1[农业科学—作物学]

 

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