氰戊菊酯和辛硫磷对大鼠中枢谷氨酸及γ-氨基丁酸免疫阳性细胞的影响  

Effects of fenvalerate and phoxim insecticides on glutamate and gamma aminobutyric acid immunoreactive cells in the central nervous system of rats

作　　者：王心如[1] 吴建平[1] 肖杭[1] 石云[1] 戴晓青[1] 何凤生[2] 

机构地区：[1]南京医科大学毒理学研究所,南京210029 [2]中国预防医学科学院劳动卫生与职业病研究所,北京100050

出　　处：《卫生研究》2000年第1期1-3,共3页Journal of Hygiene Research

基　　金：国家"九五"医学科技攻关资助项目!(No.96-906-04-11);江苏省卫生厅科技发展基金!(No.H97-16)

摘　　要：为深入探讨拟除虫菊酯和有机磷混配农药对中枢神经系统的毒性作用及其机制,通过免疫组织化学和显微图象分析方法观察了大鼠经氰戊菊酯(20m g/kgBW)、辛硫磷(160m g/kgBW)、氰戊菊酯+ 辛硫磷(180m g/kgBW,1v8等毒配比)灌胃染毒24小时后中枢神经组织中谷氨酸(Glu)和γ- 氨基丁酸(GABA)免疫阳性细胞(IRC)的变化。结果显示,氰戊菊酯组、氰戊菊酯+ 辛硫磷组大鼠大脑皮层、海马、纹状体等部位Glu的IRC数目(N)减少,阳性细胞面积比(Aa% )和积分光密度(IOD)呈现不同程度的降低,而GABA的IRC各观察参数值均呈现不同程度的升高;氰戊菊酯组与氰戊菊酯+ 辛硫磷组各参数值之间未见显著性差异;与对照组相比,单独辛硫磷组各观察参数值亦未见显著性变化。本次研究结果提示,Glu、GABA递质功能紊乱可能在拟除虫菊酯神经毒性中具有重要意义;在对Glu 和GABA作用的影响上。In order to study the neurotoxic effect and the mechanism of fenvalerate and phoxim insecticides, rats were orally treated with fenvalerate(20mg/kgBW),phoxim(160mg/kgBW)and fenvalerate (20mg/kgBW)plus phoxim (160mg/kgBW). The glutamate (Glu) and gamma aminobutyric acid(GABA)immunoreactive cells of the central nervous system of rats were observed by immunohistochemical method. The results showed that the total number, percentage of positive area and integral optical density of Glu immunoreactive cells in cerebral cortex, hippocampus and striate exhibited a characteristic decrease in the groups fed with fenvalerate and fenvalerate plus phoxim, while those of GABA were enhanced to certain extents. No obvious changes in Glu or GABA were observed in the phoxim group as compared with the controls, and no significant difference was observed between the fenvalerate group and the fenvalerate plus phoxim group. The results suggest that the disturbance of Glu and GABA metabolism may play an important role in the development of pyrethroid neurotoxicity, and there is no interaction between phoxim and fenvalerate pesticides on Glu and GABA activities.

关 键 词：氰戊菊酯 辛硫磷 GABA 中枢神经系统 

分 类 号：Q593.2[生物学—生物化学] R595.4[医药卫生—内科学]