辛硫磷对大鼠精子生成量和精子运动能力的影响  

Effects of phoxim on sperm production and motility of rats

作　　者：詹宁育[1] 王沭沂[1] 王心如[1] 

机构地区：[1]南京医科大学毒理研究所,南京210029

出　　处：《卫生研究》2000年第1期4-6,共3页Journal of Hygiene Research

基　　金："九五"国家重点科技攻关项目基金!(No.96-906-04-11);国家自然科学基金!(No.39640006)

摘　　要：为探讨有机磷杀虫剂辛硫磷的雄性生殖毒性,运用动物实验研究了不同剂量辛硫磷经口染毒大鼠60天对精子生成量和精子运动能力的影响。采用计算机辅助精子分析仪(CASA)分析精子运动轨迹。结果显示:辛硫磷24.5和73.5m g/kgBW 染毒组,大鼠睾丸精子生成量低于对照组(P< 0.05和P< 0.01)。与对照组相比,精子的运动活力参数(曲线运动速度、直线运动速度、鞭打频率)和运动方式参数(直线性和前向性)均降低(P< 0.05和P< 0.01),且具有剂量依赖关系。提示辛硫磷大剂量(24.5和73.5m g/kgBW)对雄性大鼠有生殖毒性。The effects of phoxim at various dosage levels on sperm production and motility in rats were studied. Sperm motility was measured by computer assisted sperm analysis (CASA). The results showed that rats exposed to phoxim at higher doses(24 5 and 73 5mg/kgBW) for 60 successive days showed significant reduction of daily sperm production as compared with the control (P<0 05,P<0 01). Sperm motion parameters such as currilinear velocity (VCL),straight line velocity(VSL),beat cross frequency(BCF), linearity(LIN) and straightness(STR) were significantly decreased than those of the control(P<0 05,P<0 01) which indicated a dose dependent relationship to some extent. However, there was no obvious change in body weight, testis weight and the rate of motile sperm in epididymis. It suggests that the male reproductive toxicilty of phoxim at higher doses (24 5 and 73 5mg/kgBW)mainly induces a reduction of sperm production and motility.

关 键 词：辛硫磷 精子生成量 精子运动能力 农药 生殖毒性 

分 类 号：R595.4[医药卫生—内科学] R698.202[医药卫生—临床医学]