高脂诱发小鼠Ⅱ型糖尿病模型建立及谷氨酰胺的保护作用研究  

IRDUCTION OF TYPE Ⅱ DIABETES IN MICE BY HIGH FAT DIET AND STUDY ON THE PROTECTIVE EFFECTS OF GLUTAMINE

作　　者：葛学美[1] 郭俊生[1] 赵法伋[1] 李正银[1] 陈洪章[1] 

机构地区：[1]第二军医大学军队卫生学教研室,上海200433

出　　处：《解放军预防医学杂志》2000年第1期21-24,共4页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：中国科学院生化研究所国家重点实验室资助项目

摘　　要：目的 :为揭示营养因素与糖尿病的关系提供实验依据 ,为广泛开展Ⅱ型糖尿病的实验研究提供良好的动物模型。方法 :C57BL/ 6J雄性小鼠 ,随机分为 :对照组、谷氨酰胺组、高脂 3个月后补加谷氨酰胺组、高脂组 ,实验期 5个月。结果 :高脂可使小鼠体重超常、血糖升高、血清胰岛素水平上升 ,而谷氨酰胺可阻止血糖、血清胰岛素的上升。结论 :高脂饮食诱发C57BL/ 6J小鼠产生Ⅱ型糖尿病 ,谷氨酰胺能明显下降高脂饮食所致的高血糖及高胰岛素血症 ,提示谷氨酰胺有降糖作用 ,并可改善机体胰岛素抵抗状态。Objective:This study was to provide basis for revealing fhe relationship between nutrients and diabetes and to provide a useful animal model of type Ⅱ diabetes for wide experimental study.Methods:Male C57BL/6J mice were randomly divided into four groups: control, glutamine group(Gln), high fat group (HF)and HF-Gln-L group. The experimental term was 5.5 months.Results:High fat diet could make male C57BL/6J mice overweight, hyperglycemia and hyperinsulinemia. Glutamine could reduce the levels of serum glucose and insulin. Conclusion:Hgh fat deit could induce type Ⅱ diabetes of male C57BL/6J mice. Glutamine could prevent the development of hyperglycemia and hyperinsulinemia. These results suggest that glutamine can attenuate serum glucose and reduce insulin resistance in the mice.

关 键 词：高脂 糖尿病 谷胺酰胺 

分 类 号：R587.1[医药卫生—内分泌]