TRFIA法与ELISA法检测乙肝病毒血清学标志物的比较  

Comparison of HBV Serum Marker between TRFIA and ELISA

作　　者：陈忠城[1] 凌小强[1] 林泳婷[1] 

机构地区：[1]中山大学附属第三医院检验科,广东广州510630

出　　处：《临床医学工程》2012年第3期429-431,共3页Clinical Medicine & Engineering

摘　　要：目的探讨时间分辨荧光免疫测定法(TRFIA)定量检测乙肝病毒血清学标志物(HBV-M)与酶联免疫吸附试验(ELISA)一步法定性检测HBV-M的关系及其临床意义。方法采用TRFIA和ELISA分别检测436份血清标本的HBV-M。结果对于稍高于临界值的血清,TRFIA检出率明显高于ELISA。结论 TRFIA检测HBV-M比ELISA敏感,能为乙肝的临床诊断、疗效观察提供动态数据,是一种比较理想的定量检测方法。Objective To explore the clinical value and relationship between time-resolved fluoro immtmoassay （TRFIA） to detect HBV-M in quantity and enzyme linked immtmosorbent assay （ELISA） to detect HBV-M in quality. Methods 436 serum specimen were collected to detect HBV-M by TRFIA and ELISA. Results The positive results of TRFIA were higher than thoset of ELISA for nearly cut-off value HBV-M specimen, Conclusions TRFIA is more sensitive than ELISA. It can provide the dynamic data for diagnosis and the curativ effect observation of hepatitis B, which is a perfect quantiative method.

关 键 词：乙肝病毒血清学标志物 时间分辨荧光免疫测定法 酶联免疫吸附试验 

分 类 号：R446.61[医药卫生—诊断学] R512.62[医药卫生—临床医学]