TGF-β1对肾间质成纤维细胞Ⅲ型胶原表达的影响  

Effects of TGF-β1 on cellular proliferation and expression of type Ⅲcollagen by cultured rat renal interstitial fibroblasts

作　　者：王伟铭[1] 姚建[1] 石蓉[1] 董德长[1] 

机构地区：[1]上海第二医科大学附属瑞金医院肾脏科,上海200025

出　　处：《细胞与分子免疫学杂志》2000年第1期29-31,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的通过观察TGF β1对肾间质成纤维细胞的增殖及Ⅲ型胶原mRNA表达的影响 ,探讨肾间质成纤维细胞在肾纤维化过程中的作用。方法应用大鼠肾间质成纤维细胞体外培养 ,以MTT比色法和RT PCR法 ,观察TGF β1对肾间质成纤维细胞的增殖作用和对Ⅲ型胶原mRNA表达的影响。结果TGF β1可促进肾间质成纤维细胞的增殖 ,且与TGF β1的质量浓度呈正相关 ;TGF β1可刺激肾间质成纤细胞Ⅲ型胶原mRNA的表达 ,并呈剂量和时间依赖效应。结论TGF β1与肾间质成纤维细胞的相互作用 ,在肾间质纤维化中可能起重要的作用。Aim To investigate the effects of TGF β1 on proliferation and type Ⅲcollagen mRNA expression of rat renal interstitial fibroblasts in vitro for elucidating the role of fibroblasts and TGF β1 in renal interstitial fibrosis. Methods The cells were cultured in media containing various contents of TGF β1. The Proliferation and expression of type Ⅲcollagen mRNA of the cells were assayed with MTT method and RT PCR, respectively. Results TGF β1 had a stimulating effect on proliferation with dose dependence(TGF β1 0～20 μg/L),and induced an increasing expression of type Ⅲcollagen mRNA with dose de pendence(TGF β1 0～10 μg/L)and time dependence(0～48 hours)to the renal fibroblasts. Conclusion TGF β1 is involved in proliferation of renal interstitial fibroblasts with their type Ⅲcollagen mRNA expression, and might play an important role in renal interstitial fibrosis.

关 键 词：TGF-Β1 肾间质 成纤维细胞 Ⅲ型胶原 细胞增殖 

分 类 号：R692.02[医药卫生—泌尿科学]