巴豆中巴豆苷的提取纯化及制备  

Extraction Purification and Preparation of Isoguanosine from Croton tiglium

作　　者：李生梅[1,2] 曾宝[1,2] 黄孟秋[1,2] 唐君苹[1,2] 赖小平[1,2] 林吉[1,2] 

机构地区：[1]广州中医药大学,广州510006 [2]东莞广州中医药大学中医药数理工程研究院,广东东莞523808

出　　处：《中国实验方剂学杂志》2012年第6期21-24,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部重大新药创制专项(2009ZX09103-388)

摘　　要：目的:建立一种从巴豆中提取纯化及制备巴豆苷的方法。方法:以巴豆苷得率为指标,采用正交试验设计优化巴豆苷的提取工艺参数;考察大孔树吸附脂纯化工艺条件;应用制备型液相色谱分离高纯度巴豆苷。结果:最佳巴豆苷提取工艺参数为8倍量20%乙醇提取3次,每次40 min;大孔树脂吸附初步纯化后,制备液相分离出巴豆苷单体,质量分数达98%,可作为对照品。结论:该方法简便易行,可用于分离制备高纯度巴豆苷样品。Objective： To establish a method for extracting, purifying and preparing isoguanosine from Croton tiglium. Method： Orthogonal design was used to optimize extraction technology parameters with the content of isoguanosine as index; Studying purification process of isoguanosine with macroporous resin and preparing high purity isoguanosine by preparative liquid chromatography. Result： Optimum extraction technology of isoguanosine was extracted 3 times with 8 times the amount of 20% ethanol, 40 min each time. After preliminarily purifying by macroporous resin, isoguanosine was isolated from purified extracting solution and the purity was up to 98%. Conclusion： This method was simple and easy to operate, also could be used for preprartion high purity isoguanosine samples.

关 键 词：巴豆 巴豆苷 提取 纯化 大孔树脂 制备液相 

分 类 号：R283.6[医药卫生—中药学]