西洋参茎叶总皂苷中分离纯化拟人参皂苷F_(11)工艺  

Separation and Purification Technology of Pseudo-ginsenoside F_(11) from Total Saponins in Leaves and Stems of Panax quinquefolium

作　　者：田旭[1] 任媛媛[1] 李平亚[1] 王翠竹[1] 刘金平[1] 

机构地区：[1]吉林大学再生医学科学研究所,长春130021

出　　处：《中国实验方剂学杂志》2012年第6期24-26,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省科技厅重大项目(20096039)

摘　　要：目的:优选从西洋参茎叶总皂苷中分离纯化拟人参皂苷F11的最佳工艺。方法:以拟人参皂苷F11(PF11)提取率为指标,建立HPLC-ELSD检测PF11的方法,并优选AB-8大孔吸附树脂纯化西洋参茎叶总皂苷中PF11的工艺。结果:最佳工艺为西洋参茎叶总皂苷过10倍量AB-8大孔吸附树脂柱,流速5 mL.min-1,依次用7.5 BV 30%～33%乙醇,8 BV 35%～40%乙醇进行洗脱,收集35%～40%乙醇洗脱液,回收乙醇,蒸干,得PF11粗品纯度>50.0%。结论:大孔吸附树脂分离拟人参皂苷F11方法简单、快速、可行。Objective： To optimize optimum separation and purification technology of pseudo-ginsenoside F11 from total saponins in leaves and stems of Panax quinquefolium. Method： PF11 was determined by HPLC-ELSD with yield of PF11 as index, and AB-8 type macroporous adsorption resin was choosen to purify PF11 from total saponins in leaves and stems of P. quinquefolium. Result： Optimum technology was： total saponins in leaves and stems of P. quinquefolium passed 10 times the amount of AB-8 maeroporous adsorption resin with flow rate 5 mL· min-1 , eluted with 7.5 BV 30% ethanol-33% ethanol, discard eluate, then eluted with 8 BV 35% ethanol-40% ethanol, collected eluate, recovered ethanol, evaporated, purity of pseudo-ginsenoside FH was more than 50%. Conclusion： This method of PFHwas separated by macroporous adsorption resin was simple, rapid and feasible.

关 键 词：拟人参皂苷F11 大孔吸附树脂 分离纯化 

分 类 号：R283.6[医药卫生—中药学]