硫磺熏蒸怀牛膝工艺筛选  

Screening of Sulfur Fumigation Technology for Achyranthes bidentata

作　　者：仇立志[1] 赵海霞[2] 李宝国[1] 

机构地区：[1]山东中医药大学,济南250355 [2]山东中医药大学附属医院,济南250011

出　　处：《中国实验方剂学杂志》2012年第6期35-37,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省科技厅重大科技专项(2008GG3002008)

摘　　要：目的:研究硫磺熏蒸对怀牛膝中蜕皮甾酮的影响。方法:采用高效液相色谱法测定硫磺熏蒸后蜕皮甾酮的含量,XB-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-水(15:85),检测波长250 nm,柱温25℃,流速0.8 mL.min-1。结果:硫磺用量100 g.m-3,熏蒸1次,熏蒸2 h,此时蜕皮甾酮的量最高;蜕皮甾酮在0.408～2.448μg线性关系良好,回归方程为Y=34.167X+61(r=0.999 6)。结论:硫磺熏蒸对怀牛膝中蜕皮甾酮含量有一定影响,随硫磺用量增加、熏蒸时间延长、熏蒸次数增加而呈减少趋势。Objective： To investigate influence of sulfur fumigation on ecdysterone from Achyranthes bidentata. Method： The content changes of ecdysterone after fumigation of sulfur was determined by HPLC, XB- ClS column （4.6 mm x250 mm, 5 μm）, mobile phase acetonitrile-water （15： 85）, detection wavelength 250 nm, column temperature 25℃, flow rate 0.8 mL.min-1. Result： Conditions of maximum amount of ecdysterone was ： the amount of sulfur 100 g . m -3, fumigation once for 2 h ; Linear relationship of ecdysterone in the range of 0.408-2.448 μg was good, the regression equation was Y = 34. 167X± 61 （r = 0.999 6）.Conclusion： Sulfur fumigation of ecdysterone from ecdysterone had a decreasing trends with increasing the fumigation times. A. bidentata had amount of sulfur, a certain prolonging influence, the content of exposure time and increasing

关 键 词：怀牛膝 硫磺熏蒸 蜕皮甾酮 高效液相色谱法 

分 类 号：R283.6[医药卫生—中药学]