微波协助提取在中药饮片含量测定中的应用(4)——微波法与药典法测定骨碎补中柚皮苷含量比较  

Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs(4) Comparison of Determination of Naringin from Drynaria fortunei between Microwave-assisted Extraction and Chinese Pharmacopeia Method

作　　者：方婧[1] 杨洪军[1] 付梅红[1] 张贝贝[1] 张梦阳[2] 杨雪燕[2] 张康丁 王祝举[1] 杨岚[1] 张东[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]沈阳药科大学,沈阳110016 [3]邢台医学高等专科学校,河北邢台054000

出　　处：《中国实验方剂学杂志》2012年第6期75-77,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中药生产技术及过程控制技术标准平台项目;"重大新药创制"科技重大专项(2009ZX09308-003);国家科技支撑计划子课题(2006BAI09B07-02)

摘　　要：目的:建立微波协助提取法提取骨碎补中有效成分柚皮苷含量测定方法,比较微波协助提取法与药典法提取柚皮苷优势。方法:采用Dikma Diamonsil C18色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-醋酸-水(35:4:65),检测波长283nm,柱温30℃,流速1 mL.min-1。结果:微波提取时间5 min,提取温度120℃,提取溶剂50%甲醇。柚皮苷在0.04～0.6μg呈良好线性关系,r=0.999 9。平均回收率99.51%(n=6)。结论:微波提取法较药典法提取柚皮苷更简便快速、结果准确,为一种符合环保绿色理念的含量测定方法,可用于检测骨碎补饮片中有效成分柚皮苷含量。Objective： To establish a microwave-assisted extraction method for the extraction of the naringin from Drynaria fortunei, and compare of the determination of naringin from D. fortunei between microwave- assisted extraction and Chinese pharmacopeia method. Method： Dikma Diamonsil Cls column （4.6 mm ×150 mm, 5μm） was used. The mobile phase consisted of methanol-acetic acid-water （35：4： 65）. The temperature of column was mainted at 30 ℃. The flow rate was 1.0 mL·min-1 and the detection wavelength was at 283 nm. Result： Microwave extractive time was 5 rain, the temperature was 120℃, the extraction solvent was 50% methanol. The calibration curve was in good linearity with in the range from 0.04 to 0.6 μg （r = 0. 999 9）. The average recovery was 99.51% （n = 6）. Conclusion： The method is simple, rapid, as well as precise and reliable, and can be used for the determination of naringin from D. fortunei.

关 键 词：微波提取 骨碎补 柚皮苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]