茜草提取物中大叶茜草素测定的方法研究  

The Methodology Study on Mollugin Content Determination in Radix Rubiae Extracts

作　　者：浦益琼[1] 王冰[1] 张彤[1] 陶建生[1] 丁越[1] 

机构地区：[1]上海中医药大学,上海201203

出　　处：《中国实验方剂学杂志》2012年第6期94-96,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：教育部新世纪人才计划(NCET-10-0944);上海市曙光计划(10SG40);上海市创新团队及重点学科(J50302)

摘　　要：目的:建立茜草提取物中大叶茜草素的HPLC含量测定方法。方法:采用Ultimate XB-C18(4.6 mm×250 mm,5μm),流动相为甲醇-乙腈-0.2%磷酸(25:50:25),流速为1 mL.min-1,柱温25℃,检测波长250 nm。结果:在该条件下,大叶茜草素在3.566～456.4 mg.L-1线性关系良好(r=0.999 9);平均回收率为98.97%(RSD 2.29%)。结论:该方法准确、可靠,操作简便,可用于茜草提取物的质量控制方法。Objective： To establish a quantitative HPLC method for the determination of mollugin in extract from Rubia cordifolia L. Method： The determination was carried out on a Ultimate XB-C18 column at 25℃with methanol-acetonitrile-0. 2% phosphoric acid （25： 50： 25） as mobile phase and detection wavelength at 250 nm. The flow rate was 1.0 mL·min-l. Result： The good linearity was obtained with the correlation coefficients ranged of 3. 566-456.4 mg · L-1 （r =0.9999）. The average recovery rate was 98.97% （RSD 2.29%）. Conclusion： This method is accurate and reliable with easy operation, which can be used in the quality control of extracts from R. cordifolia.

关 键 词：茜草 大叶茜草素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]