郁心效的抗抑郁作用及其机制研究  

Preliminary Study on Antidepressant Effect of Yuxinxiao and its Mechanism

作　　者：王瑜[1] 钱令嘉[2] 赵云[2] 潘勤 石卿[4] 张岭[4] 

机构地区：[1]天津中医药大学,天津300193 [2]解放军军事医学科学院,北京100850 [3]天津中新药业股份有限公司,天津300193 [4]河北联合大学,河北唐山063009

出　　处：《中国实验方剂学杂志》2012年第6期129-132,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家973课题计划项目(2011CB505106)

摘　　要：目的:确认郁心效的抗抑郁作用,并初步探讨其抗抑郁作用的机制。方法:小鼠随机分5组:模型组、阳性药物对照组、郁心效低、中、高剂量(320,640,1 280 mg.kg-1)组,均ig 7 d,于末次给药后建立5-羟色氨酸(5-HTP)诱导小鼠甩头和育亨宾小鼠致死增强模型,观察郁心效对5-HTP(10 mg.kg-1,iv)诱导小鼠甩头行为和对小鼠皮下注射(sc)育亨宾(35 mg.kg-1)致死增强的影响;大鼠随机分6组:正常组、模型组、盐酸舍曲林(10 mg.kg-1)组、郁心效低、中、高剂量(220,440,880mg.kg-1)组,建立慢性不可预知性温和应激模型(CUMS),造模同时ig给药8周,观察郁心效对大鼠糖水偏嗜度、旷场行为和海马中5-羟色胺(5-HT),多巴胺(DA),去甲肾上腺素(NE)含量的影响。结果:郁心效可显著增加5-HTP诱导的小鼠甩头次数,对育亨宾诱导的小鼠致死量没有显著影响;可显著提高CUMS大鼠的糖水偏嗜度、旷场行为总评分和海马中5-HT,DA的含量。结论:郁心效具有明确的抗抑郁作用,其作用机制可能与5-HT系统有关。Objective： To study the antidepressant effect and mechanism of Yuxinxiao. Method： Mice were randomly divided into five groups as follows： the model group, positive control group, Yuxinxiao groups （320, 640, 1 280 mg ·kg-1 ）, all groups were administered ig for 7 days, 5-hydroxytryptophan （5-HTP） - induced head-twitch and yohimbine-induced lethality tests were used after the last administration to observe the impact of 5-HTP （10 mg · kg-1, iv） induced head twitch behavior and yohimbine-induced （35 mg ·kg-1, sc） lethality in mice; rats were randomly divided into control group, model group, sertraline hydrochloride group （10 mg ·kg-1 ） , Yuxinxiao groups （220, 440, 880 mg ·kg-1 ）. The chronic unpredictable mild stress model was established by continuous garage 8 weeks, the sucrose preference degree, open-field behavior and the content of 5- HT, dopamine （DA） and norepinephrine （NE） in hippocampus were tested to evaluate the empact Of Yuxinxiao on CUMS models. Result： Yuxinxiao （320, 640, 1 280mg ·kg-1, ig） ignificantly increased the frequenee of 5-HTP （ 10 mg ·kg-1, iv） induced head-twitch in mice after 7 days of continuous administrations while it did not enhance the yohimbine-induced （35mg ·kg-1, sc） lethality in mice. increased remarkably in hippocampus of the model group treated with high after 8 weeks of continuous administrations compared with model groups. antidepressant effect with manner of dose-dependent. The meehanism may central nervous system. The contents of 5-HT and DA were dose of Yuxinxiao （880mg ·kg-1） Conclusion： Yuxinxiao has a clear be related to the system of 5-HT in

关 键 词：郁心效 抗抑郁作用 小鼠甩头实验 育亨宾致死增强实验 慢性不可预知温和应激抑郁模型 

分 类 号：R285.5[医药卫生—中药学]