黄芩素对4种肝损伤物质造成肝细胞毒性的拮抗作用  

Protection of Baicalein against Four Hepatotoxins-induced Cytotoxicity

作　　者：蒋萍[1] 杨阳[1] 季莉莉[1,2] 王峥涛[1,2] 

机构地区：[1]上海中医药大学中药研究所中药标准化教育部重点实验室,中药新资源与质量标准综合评价国家中医药管理局重点研究室,上海201210 [2]上海中药标准化研究中心,上海201210

出　　处：《中国实验方剂学杂志》2012年第6期145-148,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：上海市科技启明星计划(跟踪)(10QH1402200);国家自然科学基金项目(青年基金30801544)

摘　　要：目的:观察黄芩素对4种肝毒性物质诱导的人正常肝细胞株L-02细胞损伤的拮抗作用。方法:培养的L-02细胞分别给予10 mmol.L-1对乙酰氨基酚(AP),50μmol.L-1山岗橐吾碱(clivorine,CLI),2μmol.L-1川楝素(toosendanin,TSN)和100 mmol.L-1乙醇(EtOH)诱导肝细胞毒性。黄芩素1,10,25,50,100μmol.L-1分别与细胞预孵15 min后,加入肝毒性物质,48 h后MTT法检测细胞存活率。结果:与对照组相比,4种肝毒性物质均能显著降低肝细胞存活率(P<0.001)。经不同浓度黄芩素处理后,各浓度黄芩素均能显著提高AP,TSN,EtOH肝细胞损伤模型组的细胞存活率(P<0.01,P<0.001);10,25,50,100μmol.L-1黄芩素能显著提高CLI肝细胞损伤模型组的细胞存活率(P<0.05,P<0.001)。结论:黄芩素具有拮抗对乙酰氨基酚、山岗橐吾碱、川楝素和乙醇这些肝毒性物质诱导的肝细胞毒性的作用,且具有一定的浓度依赖性。Objective： The present study is designed to observe the protective effects of baicalein against hepatotoxins induced cytotoxicity on the human normal liver L-02 cells. Method： L-02 cells were seeded and pretreated with or without baicalein （1, 10, 25, 50, 100μmol·L-1） for 15 min, and then cells were further treated with or without various hepatotoxins, including acetaminophen （AP, 10 mmol· L-l） , clivorine （CLI, 50μmol·L-1）, toosendanin （TSN, 2 μmol·L-1） and ethanol （EtOH, 100μmol·L-1） for 48 h. The survival ceils were determined by MTT assay. Result： Four hepatotoxins could significantly reduce the cell viability （P 〈 0. 001） compared with the control group. After treatments, baicalein could increase the cell viability which was decreased by AP, TSN and EtOH （P 〈 0.01, P 〈 0. 001 ）. Moreover, baicalein could significantly increase the cell viability which was decreased by CLI （P 〈 0.05, P 〈 0. 001 ）. Conclusion： Baicalein can prevent the cytotoxicity induced by those hepatotoxins in human normal liver L-02 cells.

关 键 词：黄芩素 肝毒性 肝细胞 

分 类 号：R285.5[医药卫生—中药学]