淫羊藿苷对大鼠颈总动脉内膜损伤后骨桥蛋白表达的影响  

The Effects of Icariin on Expression of OPN mRNA after Vascular Injury in Rats

作　　者：高爱社[1] 苗莉 沈晓君[1] 

机构地区：[1]河南中医学院,郑州450008 [2]河南武警总队医院,郑州450006

出　　处：《中国实验方剂学杂志》2012年第6期163-165,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省教育厅自然科学基金项目(2007310005);河南科技攻关计划项目(0624420026)

摘　　要：目的:研究淫羊藿苷对大鼠颈动脉内膜损伤后新生内膜内骨桥蛋白表达的影响。方法:取大鼠42只,随机分为正常对照组6只,模型组和淫羊藿苷高、低剂量组(120,60 mg.kg-1)各12只。后3组建立鼠颈动脉球囊损伤术后狭窄模型,淫羊藿苷组术前6 d开始给予淫羊藿苷灌胃至实验结束;模型对照组以等体积生理盐水灌胃。术后14 d和28 d分批处死动物。取各组实验动物颈动脉损伤段,常规石蜡切片,免疫组织化学S-ABC法检测骨桥蛋白(OPN)的表达;RT-PCR检测OPNmRNA的表达。结果:在球囊损伤后14,28 d时,淫羊藿苷高、低剂量组OPN mRNA的表达14 d时为(0.58±0.01),(0.42±0.02),28 d时为(0.28±0.02),(0.33±0.03),明显低于模型组14 d(0.71±0.02),28 d(0.62±0.01);高、低剂量组之间无明显差异。淫羊藿苷高、低剂量OPN蛋白表达量14 d时为(20.21±3.67),(32.53±12.16),28 d时为(26.14±4.64),(24.16±11.35),明显低于模型组14 d(71.24±13.25),28 d(46.13±12.31),均P<0.01。结论:淫羊藿苷在动脉损伤后可调控OPN的表达,抑制平滑肌细胞的迁移,从而减轻球囊损伤后新生内膜的增生。Objective： To observe the effect of Icariin on the levels of osteopontin （OPN） in rat carotid artery injured by balloon and to evaluate the mechanisms of inhibiting intima hyperplasia. Method： Fourty-two rats were randomly divided into 4 groups： control group, model group and icariin 60 mg. kg-1group and icariin 120 mg . kg-1 group. Rat carotid balloon injury restenosis model was established. Rats of icariin group were treated with drugs for 2 or 4 weeks. The expression of OPN mRNA were assessed by RT-PCR and immunohistochemistry strategy analysis. Result- Compared with model group, the icariin group significantly decreased the expression of OPN at 14 and 28 day after injury （P 〈 0. 01 ）. The difference between two doses was not significant. Conclusion ： Icariin could inhibit neointimal proliferation of rat carotid artery injured by balloon. The mechanism might be related to the effects of icariin for decreasing level of OPN, and inhibiting the migration and proliferation of vascular smoothmuscle cells.

关 键 词：淫羊藿苷 新生内膜 骨桥蛋白 

分 类 号：R285.5[医药卫生—中药学]