蛇床子-补骨脂在乳腺癌骨转移裸鼠模型中吸收入血特征的研究  

Study on Characteristics of Absorption into Blood of Common Cnidium Fruit-Malaytea Scurfpea Fruit in Breast Cancer Bone Metastasis Nude Mice Model

作　　者：程旭锋[1,2] 刘胜[2] 金惠[3] 张新峰[4] 刘琦5 

机构地区：[1]河南中医学院第一附属医院乳腺外科,郑州450000 [2]上海中医药大学附属龙华医院乳腺科,上海200032 [3]华东理工大学生物反应器国家重点实验室&药学院,上海200237 [4]河南省肿瘤医院中西医结合科,郑州450008

出　　处：《中国实验方剂学杂志》2012年第6期175-180,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30772811);河南省教育厅自然科学基金项目(2010B360005);郑州市科技局科技攻关计划(112PPTSF319-14);郑州市金水区技术研究与开发经费支持项目(20093542)

摘　　要：目的:以蛇床子素和补骨脂素为靶标成分,从药物吸收入血角度探讨蛇床子-补骨脂配伍后疗效增加的机制。方法:采用左心室注射MDA-MB-231BO细胞法建立裸鼠乳腺癌骨转移模型,给予不同配比的蛇床子-补骨脂药物煎液,采用HPLC-MS法测定裸鼠血浆中蛇床子素和补骨脂素,观察蛇床子-补骨脂配伍后蛇床子素和补骨脂素吸收入血的特征。结果:蛇床子组血浆中可发现蛇床子素离子峰;补骨脂组血浆中可发现补骨脂素分子及其离子峰信号;蛇床子-补骨脂(1:3)组血浆中可发现蛇床子素分子及离子峰信号,未发现补骨脂素分子及离子峰信号,蛇床子-补骨脂(2:2)组可发现蛇床子素和补骨脂素的分子及离子峰信号,蛇床子-补骨脂(3:1)组可发现蛇床子素分子及离子峰信号,未发现补骨脂素分子及离子峰信号。结论:蛇床子素和补骨脂素均可被吸收入血;蛇床子与补骨脂1:1配伍可促进补骨脂中补骨脂素的吸收入血。Objective： To investigate the mechanism of curative effect increased of Common Cnidium Fruit-Malaytea Scurfpea Fruit compatibility based on osthole and psoralen as the target components absorbed into the bloodstream. Method： The breast cancer bone metastasis nude mice models were established by injecting MDA- MB-231BO cells into left ventricular, and they were given different combinations of Common Cnidium Fruit- Malaytea Scurfpea Fruit drugs decoction, osthole and psoralen in nude ice plasma were detected by HPLC-MS, and the characteristics of osthole and psoralen absorbed into the bloodstream were observed. Result： The plasma osthole＇s ion peak could be found in the plasmas of Common Cnidium Fruit group; the psoralen molecule and its ion peak signal could be found in the plasmas of Malaytea Scurfpea Fruit group; osthole molecule and its ion peak signal, but not psoralen molecule or its ion peak signal could be found in the plasmas of Common Cnidium Fruit- Malaytea Scurfpea Fruit （1： 3） group ; not only osthole molecule and its Ion peak signal but also psoralen molecule and its lon peak signal could be found in the plasmas of Common Cnidium Fruit-Malaytea Scurfpea Fruit （2： 2） group; osthole molecule and its ion peak signal, but not psoralen molecule or its ion peak signal could be found in the plasmas of Common Cnidium Fruit-Malaytea Scurfpea Fruit （3：1 ） group. Conclusion： Osthote and psoralen could be absorbed into the bloodstream, and the absorption into bloodstream of psoralen could be promoted by Common Cnidium Fruit and Malaytea Scurfpea Fruit 1：1 compatibility.

关 键 词：蛇床子 补骨脂 乳腺癌骨转移裸鼠模型 

分 类 号：R284.1[医药卫生—中药学]