补肾疏肝方对Lewis肺癌荷瘤小鼠免疫及内分泌的影响  

Effect of Bushen Shugan Formula on Immune Function and Endocrine System of Lewis Lung Cancer Mice

作　　者：古兆森[1] 王淑玲[2] 刘源[1] 

机构地区：[1]郑州大学第一附属医院中西医结合科,郑州450052 [2]郑州大学基础医学院病理生理学科,郑州450001

出　　处：《中国实验方剂学杂志》2012年第6期188-191,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省科技厅科技攻关项目(112300410049)

摘　　要：目的:研究补肾疏肝方对小鼠Lewis肺癌的抑制作用并探讨可能存在的免疫及内分泌机制。方法:选取荷瘤成功的小鼠40只,随机分为4组,分别为模型组、顺铂组、中药组、综合组。分别给予生理盐水(0.4 mL)、顺铂(0.5 mL,0.2 g.L-1)、补肾疏肝方中药(0.4 mL,1.75 g.L-1)、中药联合顺铂干预14 d。于第15天摘眼球取血、剥取肿瘤称质量、计算抑瘤率。采取酶联免疫吸附(ELISA)的方法检测血清白细胞介素-2(IL-2)、白细胞介素-10(IL-10)、雌二醇(E2)、睾酮(T)含量。结果:中药组、综合组、顺铂组抑瘤率及肺转移灶抑制率方面均显著高于模型组,对比具有统计学意义(P<0.05);中药组、顺铂组、综合组荷瘤小鼠血清IL-2,T水平升高;血清IL-10,E2水平降低,与模型组比较均有意义(P<0.05)。结论:补肾疏肝方可以抑制肿瘤生长,降低肺转移灶数目;具有调节免疫细胞因子IL-2,IL-10的作用,从而提高荷瘤小鼠的免疫力;可以调节荷瘤小鼠内分泌环境,增强小鼠抗肿瘤的作用。Objective： To investigate the effect of Bushen Shugan （BSSG） formula on immune function and endocrine system of Lewis lung cancer mice and discuss the possible mechanism. Method： The mice transplanted Lewis lung cancer were randomLy divided into model control group, BSSG group, BSSG combined with cisplatin （DDP） group and DDP group. After two weeks＇ treatment, the mice were sacrificed and blood was taken from eyeballs, and the tissue of tumor was taken and weighted. The concentration of serum interleukin-2 （IL-2） ,.IL-10, estradiol （E2） and testosterone （T） were measured by enzyme-linked immuno sorbent assay （ELISA）. Result： Compared with the control group, the BSSG group can inhibit mouse tumor growth and lung cancer metastasis. The content in blood serum of IL-2, T, E2 of BSSG group, DDP group and BSSG combined with DDP group is higher than that of model control group respectively. The level of IL-10 in blood serum of them is lower than model control group respectively. Conclusion： BSSG formula can suppress tumor growth and decrease the number of lung metastatic tumors in the mice ; BSSG formula can make an effect on the level of IL-2, IL-10, E2 and T. It can improve the functions of immunity and regulate internal secretion.

关 键 词：补肾疏肝方 LEWIS肺癌 白细胞介素-2 白细胞介素-10 雌二醇 睾酮 

分 类 号：R285.5[医药卫生—中药学]