板蓝根抗炎作用有效部位初步筛选  

Preliminary Screening of Anti-inflammatory Fraction of Isatidis Radix

作　　者：陈凯[1] 窦月[1] 孟凡刚[1] 田源[1] 陈智[2] 田景振[1] 

机构地区：[1]山东中医药大学药学院,济南250355 [2]山东省济南市第二人民医院药剂科,济南250022

出　　处：《中国实验方剂学杂志》2012年第6期200-203,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省重大创新药物综合平台-中药创新药物平台(2009ZX09301-013)

摘　　要：目的:分离并初步筛选板蓝根抗炎作用有效部位。方法:通过膜分离将板蓝根总提取物分为不同截留相对分子质量段,采用二甲苯致小鼠耳肿胀初步筛选,再将有效相对分子质量段用大孔树脂、离子交换树脂分离得到不同部位进一步采用二甲苯致小鼠耳肿胀、小鼠腹腔毛细血管通透性评价活性,最后通过特殊颜色反应确定有效部位化学性质。结果:板蓝根中相对分子质量小于1 000的物质对二甲苯致小鼠耳肿胀模型抑制率为40.29%,与空白组相比有显著性差异(P<0.05)。板蓝根小于1 000 D物质中被732阳离子交换树脂吸附部分对二甲苯致小鼠耳肿胀模型抑制率为28.20%,对醋酸致小鼠毛细血管通透性改变模型抑制率为17.76%,与空白组相比均有显著性差异(P<0.05)。颜色反应说明该部位主要为氨基酸类及生物碱类成分。结论:板蓝根总氨基酸、总生物碱部位可能是其抗炎有效部位。Objective： To separate and screen anti-inflammatory fraction of Isatidis Radix. Method： Membrane separation method was used to separate Isatidis Radix into different sections, their anti-inflammatory effects were evaluated by acetic acid-induced celiac capillary permeabil!ty and xylene-induced mouse ear swelling models in mice. Macroporous resin and ion exchange resin were used to further separate Isatidis Radix into different sections, and the anti inflammatory effects were evaluated by abouve mentioned models, finally, their chemical property was determined by color reactions. Result： The substance less than 1 000 in Isatidis Radix had a good inhibiting effect in mice era swelling model, the inhibition rate of swelling being 40.29% （P 〈 O. 05）. The fraction absorbed by 732 cationic resin in the less than 1 000 substances could inhibit celiac capillary permeability andear swelling, the inhibiting rate being 17.76% and 28.20% , respectively （P 〈 0.05）. Color reactions showed that the fraction was composed of amino acids and alkaloids. Conclusion ： The amino acids and alkaloids of Isatidis Radix could be the anti-inflammatory fraction.

关 键 词：板蓝根 抗炎作用 有效部位 膜分离 

分 类 号：R285.5[医药卫生—中药学]