明党参根皮中5种呋喃香豆素类成分的体外抗肿瘤活性  

Study on Antitumor Activity of Five Furanocoumarins from the Root Bark of Changium smyrnioides in vitro

作　　者：王萌[1] 陈建伟[1] 李祥[1] 

机构地区：[1]南京中医药大学药学院,南京210046

出　　处：《中国实验方剂学杂志》2012年第6期203-205,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏高校优势学科建设工程项目;高等学校博士学科点专项科研基金(200803150009);江苏省自然科学基金项目(BK2003107)

摘　　要：目的:研究明党参根皮中异欧前胡素、欧前胡素、花椒毒酚、珊瑚菜内酯、5-羟基-8-甲氧基补骨脂素5种呋喃香豆素类成分的体外活性。方法:选取人肝癌细胞株SMMC-7721,HepG2,人肺癌细胞株A-549,人胃癌细胞株MKN-45,人宫颈癌细胞株Hela,人乳腺癌细胞株MCF-7,MDA-MB-2317种肿瘤细胞。以5×104个/mL的细胞密度接种于96孔板,给予不同浓度的药物后培养72 h,采用MTT法观察5种成分对各细胞活性的影响。结果:5种呋喃香豆素对7种肿瘤细胞均表现出不同程度的增殖抑制作用,IC50在0.30～17.23 mg.L-1之间,其中异欧前胡素效果最显著,IC50从0.39 mg.L-1到4.11 mg.L-1。结论:明党参根皮中5种呋喃香豆素类成分具有明显的抗肿瘤活性。Objective： To study the in vitro activity of five furanocoumarins from the root bark of Changium smyrnioides Wolff. isoimperatorin, imperatorin, xanthotoxol, phellopterin and 5-hydroxyl-g-methoxy- psoralen. Method： The antitumor effect against human liver cancer （SMMC-7721 and HepG2）, human lung cancer （A549）, human gastric cancer （MKN45）, human uterine cervix cancer （Hela） and human breast cancer（ MCF-7 and seven kinds MDA-MB-231 ） was observed by MTT of tumors. IC50 was from 0. 30 mg . L Result： Five furanocoumarins had different inhibition on to 17.23 mg. L-1, especially isoimperatorin was most significantly effective. IC50 was from 0. 39 mg . L-1 to 4. 11 mg. L-1. Conclusion： These five furanocoumarins have obvious antitumor effect.

关 键 词：明党参根皮 呋喃香豆素 肿瘤细胞 抑制作用 

分 类 号：R285.5[医药卫生—中药学]