麝香乌龙丸对佐剂性关节炎大鼠滑膜组织病理形态及TNF-α,IL-6表达的影响  

Effect of Shexiang Wulong Wan on the Pathomorphology and Expresssion of TNF-α and IL-6 in Synovium in the Rats with Adjuvant Arthritis

作　　者：陈守中[1] 袁强[1] 张爱国[1] 王鹏飞[1] 易永泽[1] 王志文[1] 

机构地区：[1]河北联合大学附属医院,河北唐山063000

出　　处：《中国实验方剂学杂志》2012年第6期234-236,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察麝香乌龙丸对佐剂性关节炎(AA)大鼠滑膜组织病理形态及滑膜组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)表达的影响。方法:将40只雄性Wistar大鼠随机分为正常组(A)10只,造模组30只。造模组大鼠于右后足跖皮下注射完全弗氏佐剂(CFA)0.1 mL造成AA模型。将造模成功的造模组随机分为模型组(B)、麝香乌龙丸低、高剂量组(C,D,n=10),ig给予1.0,2.0 g.kg-1的麝香乌龙丸,每日1次;A,B组每日ig生理盐水2 mL/只。30 d后,取跖趾关节,HE染色观察滑膜组织病理形态,免疫组化法检测滑膜组织中TNF-α,IL-6的表达。结果:麝香乌龙丸低、高剂量组滑膜组织破坏程度较模型组明显减轻;TNF-α,IL-6在麝香乌龙丸低、高剂量组中表达均较模型组显著降低(P<0.01或P<0.05),麝香乌龙丸低、高剂量组比较无统计学意义。结论:麝香乌龙丸能改善AA大鼠滑膜组织病理损害,下调TNF-α,IL-6的表达。Objective： To observe the effects of Shexiang Wulong Wan （SXWLW） on the pathomorphology and tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6） in synovium rats with adjuvant arthritis. Method： Adjuvant arthritis （AA） animal model was reproduced. Forty Wistar male rats were divided randomly into 4 groups, model group, normal group treated with normal saline （NS） of 2 mL every day; SXWLW low dose group treated with 1.0 g . kg -1 every day; SXWLW high dose group treated with 2.0 g . kg-1 every day. At the end of the experiment, toe metatarsal joints were collected for pathological examination. The level of TNF-α and IL-6 was checked by immunohistochemical methed. Result： SXWLW low dose and high dose groups, compared with the model group, synovial tissue of damage was significantly reduced; the expression of TNF-α and IE-6 in the SXWLW low dose group and high dose group, compared with model group, decreased （P 〈 0.01 or P 〈 0.05）. Conclusion： SXWLW can reduce the expression of TNF-α and IL-6 in the synovial tissue, therefore it could decrease synovial tissue damage.

关 键 词：麝香乌龙丸 佐剂性关节炎 类风湿关节炎 肿瘤坏死因子-α白细胞介素-6 

分 类 号：R285.5[医药卫生—中药学]