急性胰腺炎时环氧化酶-2表达与腺泡细胞凋亡  

Cox-2 expression and apoptosis of acinar cells in rats with acute pancreatitis

作　　者：谢荣俊[1] 张树友[1] 李峰[1] 周筱筠[1] 刘龙飞[1] 卢先州[1] 

机构地区：[1]南华大学附属南华医院普通外科,湖南衡阳421001

出　　处：《中国现代医学杂志》2012年第5期15-18,共4页China Journal of Modern Medicine

基　　金：湖南省卫生厅资助项目(No:B2007115)

摘　　要：目的探讨急性胰腺炎(AP)时环氧化酶-2(Cox-2)表达与腺泡细胞凋亡的关系。方法 SD大鼠30只,随机分为假手术组(A组)、急性水肿性胰腺炎组(B组)和急性出血坏死性胰腺炎组(C组)。采用逆行胰胆管穿刺注射法复制急性水肿性与出血坏死性胰腺炎模型。检测造模后12 h各组的血清淀粉酶活性、腹水量、胰腺组织大体病理改变及评分、镜下病理改变与评分、腺泡细胞凋亡指数(AI)及Cox-2蛋白表达情况。结果①C组血清淀粉酶活性、腹水量、大体及镜下病理评分、Cox-2阳性率显著高于B组(P<0.01),AI显著低于B组(P<0.01)。②B、C组中AI与大体及镜下病理评分均呈显著负相关关系(P<0.05)。③B、C组中Cox-2表达与AI均呈显著负相关关系(P<0.01)。结论 AP时腺泡细胞凋亡是机体的一种自我保护机制,Cox-2过度表达可能通过抑制腺泡细胞凋亡加重胰腺病变。[Objective] To explore the relationship between Cox-2 expression and apoptosis of acinar cells in rats with acute pancreatitis. [Methods] Thirty SD rats were randomly divided into three groups： group A （shame operation group）, group B （acute edematous pancreatitis group） and group C （acute hemor- rhagic necrotic group）. Two kinds of pancreatitis model were induced by retrogradely injection of sodium de- oxycholate. After 12 hours, the serum amylase activity, ascites, apoptotic index （AI） of acinar cells and ex- pression of Cox-2 were observed, the general and histological changes of pancreas were evaluated by the cor- responding score system. [Results] The serum amylase activity, ascites volume, general and histological pathology score, positive rate of Cox-2 in group C were significantly higher than B （P〈0.01）, AI lower than B （P〈0.O1）. In group B and C, AI were negatively correlated with general and histological pathology score （P〈0.05）. In group B and C, the expressions of Cox-2 were negatively correlated with AI （P〈0.01）. [Conclusions] The apoptosis of acinar cells is a self-protection mechanism of individuals with AP, Cox-2 inhibiting the apoptosis of acinar cells and deteriorate pancreatic disease.

关 键 词：急性胰腺炎 环氧化酶-2 腺泡细胞凋亡 

分 类 号：R657.51[医药卫生—外科学]