水通道蛋白-1在急性肺损伤中的表达及意义  

Expression of AQP-1 in acute lung injury induced by lipopolysacharide in rats

作　　者：王晴[1] 刘正娟[1] 马乐[1] 赵永利[1] 白雪梅[1] 

机构地区：[1]大连医科大学附属第二医院儿科,辽宁大连116027

出　　处：《大连医科大学学报》2012年第1期22-25,共4页Journal of Dalian Medical University

基　　金：辽宁省教育厅高校科研项目(L2010119)

摘　　要：[目的]探讨水通道蛋白1(AQP-1)在脂多糖(LPS)所致的急性肺损伤(ALI)中的作用。[方法]根据是否腹腔注射脂多糖(LPS)将实验动物分为脂多糖组(LPS组)及生理盐水对照组(Con组),对比两组动物组织病理学改变、动脉血气、肺湿/干重比及AQP-1表达的差异。[结果]光镜下见LPS组肺组织水肿,大量炎性细胞浸润;LPS组PO2(55.07±17.02)mmHg明显低于Con组(97.35±9.37)mmHg,差异有显著性意义,P<0.05;LPS组肺湿/干重比(4.93±0.16)明显高于Con组(4.11±0.69),差异有显著性意义,P<0.05;免疫组化染色显示:LPS组的AQP-1蛋白表达减弱,荧光实时定量PCR证实LPS组的AQP-1 mRNA表达较对照组明显下降(P<0.01)。[结论]AQP-1参与了急性肺损伤的形成。[Objective]To investigate the expression and effect of AQP-1 in acute lung injury（ALI） induced by lipopolysaccharide.[Methods]Twenty Sprague-Dawley rats were randomly divided into LPS group treated with LPS injection and normal control group injected with saline（Control group）.Arterial oxygen tension（PaO2） and wet/dry mass ratio（W/D） were tested at 6th hour after the injection.The expressions of AQP-1 protein in the lungs of rats were detected by immunohistochemistry and the expressions of AQP-1 mRNA were measured by a real-time fluorescence PCR.[Results]The arterial oxygen tension（PaO2） in LPS group was obviously lower than that in control group（55.07±17.02） mmHg vs（97.35±9.37） mmHg（P0.05）.The wet/dry mass ratio（W/D） in LPS group was obviously higher than that in control group（4.93±0.16 vs 4.11±0.69,P0.05）.The immunostaining showed a significant decrease in the expression of AQP-1 and a real time RT-PCR confirmed that the levels of AQP-1 mRNA were significantly reduced in LPS group（P0.01） at 6 h after LPS injection. [Conclusion]AQP-1 has an great effect in acute lung injury（ALI）.

关 键 词：急性肺损伤 脂多糖 水通道蛋白-1 

分 类 号：R563.8[医药卫生—呼吸系统]