Exendin-4对IL-1β诱导小鼠胰岛β细胞凋亡的保护作用  

Protective Effect of Exendin-4 on Interleukin-1β-induced Apoptosis in Murine Pancreatic β-cells

作　　者：刘晓红[1] 王燕萍[1] 王林曦[1] 刘小莺[1] 刘礼斌[1] 

机构地区：[1]福建医科大学附属协和医院内分泌研究所,福州350001

出　　处：《福建医科大学学报》2012年第1期7-10,共4页Journal of Fujian Medical University

基　　金：福建省自然科学基金(2010J01162);福建省卫生厅创新课题(2009-CX-4);福建省卫生厅青年基金(2009-2-27)

摘　　要：目的探讨GLP-1受体激动剂Exendin-4对细胞因子IL-1β诱导的小鼠胰岛β细胞株MIN6细胞凋亡的保护作用。方法体外培养小鼠胰岛β细胞株MIN6细胞,用不同浓度(5,10,20,40ng/mL)IL-1β作用24h,噻唑兰还原(MTT)法观察不同浓度IL-1β作用后细胞存活率;Hoechst-PI荧光染色法观测细胞凋亡形态;后以Exendin-4(100nmol/L)预处理细胞24h,观察Exendin-4对IL-1β诱导的MIN6细胞凋亡的保护作用。结果 MTT显示IL-1β(5～40ng/mL)作用24h后,MIN6细胞的增殖率明显下降,并呈现一定的量效关系;Hoechst-PI染色荧光显微镜检测证实随着IL-1β浓度的增高,MIN6细胞的凋亡率亦增加。Exendin-4预先处理24h后可以抑制IL-1β诱导的β细胞凋亡。结论 GLP-1受体激动剂Exendin-4可以显著抑制IL-1β诱导MIN6细胞的凋亡,提示Exendin-4对IL-1β诱导的MIN6细胞的凋亡具有保护作用。Objective In the present study murine MIN6 pancreatic β-cells were employed to explore the protective effect of Exendin-4 on IL-1 β-induced apoptosis.Methods Murine MIN6 pancreatic β-cells were first treated with or without Exendin-4（100 nmol/L） for 24 hours,then they were incubated with different concentration of IL-1β（5,10,20,40 ng/mL）for another 24 hours.Cell viability was determined by MTT assay,and cells undergoing apoptosis were determined by fluorescence microscopy after Hoechst33342-propidium iodide staining.Results The proliferation of MIN6 cells was significantly inhibited after exposure to IL-1β with the concentrations ranging from 5 to 40 ng/mL for 24 h.Cell viability declined significantly（P0.01 compared with control group） when treated with IL-1β with the concentration over 10 ng/mL for 24 h.Pretreatment with Exendin-4,a long-acting GLP-1 receptor agonist,at the concentration 100 nmol/L for 24 h prior to exposure to IL-1β protected cells from IL-1β-induced apoptosis.Conclusion The results of our study indicate that Exendin-4 plays a protective role against IL-1β-induced apoptosis in MIN6 pancreatic β-cells.

关 键 词：白细胞介素1Β 胰岛 细胞凋亡 氨基酸类 毒液类 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R341.7[医药卫生—基础医学]