防御素2在肝内胆管结石病中的抗菌作用  

Anti-bacterial Effects of HBD2 in Intrahepatic Bile Duct of Calculus

作　　者：陈辉星[1] 洪海杰[1] 朱金海[1] 韩圣华[1] 周良艺[1] 唐南洪[1] 陈燕凌[1] 

机构地区：[1]福建医科大学附属协和医院肝胆外科,福州350001

出　　处：《福建医科大学学报》2012年第1期28-30,35,共4页Journal of Fujian Medical University

基　　金：福建省卫生厅青年基金(2007-2-26)

摘　　要：目的探讨人防御素2(HBD2)在肝胆管结石病中的抗菌作用。方法应用免疫组织化学方法和RT-PCR检测肝内胆管结石患者手术切除肝组织标本HBD2的表达情况。比较胆汁培养为革兰阴性及阳性菌组的HBD2表达的强弱,左右肝组织HBD2表达的强弱,分析HBD2表达与急性生理功能和慢性健康状况评分系统(ARACHEⅡ)评分的关系。结果 HBD2在部分肝内胆管结石的胆管间质细胞胞浆中表达,革兰阴性菌组的肝组织HBD2表达强于革兰阳性菌组,左肝部位HBD2的表达强于右肝部位,ARACHEⅡ高分组的HBD2表达强于低分组。结论 HBD2在肝胆管结石病肝组织中起到抗菌作用,其表达强度与革兰阴性菌和ARACHEⅡ评分正相关。Objective To investigate the function of the Human β-defensin 2（HBD2） in human intrahepatic bile duct of calculus.Methods HBD2 expression was studied by immunohistochemistry and RT-PCR in 60 cases of human intrahepatic bile duct of calculus.Meanwhile,the bile bacterial culture profiles for each patient were documented,and the Acute Physiology and Chronic Health Evaluation II（APACHE II） score for each case at the time of diagnosis of intrahepatic bile duct of calculus was recorded.Result The expression of HBD2 in patients with Gram-negative bacteria culture was stronger than patients with Gram-positive bacteria culture.The difference was statistically significant（17/36∶4/24,P0.05）.HBD2 expression level was higher in the left part of liver.Patients with high APACHE II score showed stronger HBD2 expression than those with low APACHE II score and the difference was statistically significant.Conclusion HBD2 is expressed in the tissue of human intrahepatic bile duct of calculus,and its expression level strongly correlates with Gram-negative bacteria found and a high APACHE II score.

关 键 词：Β防御素 胆管 肝内 胆管疾病 胆结石 肝 免疫活性 

分 类 号：R657.4[医药卫生—外科学]