COI基因作为丛赤壳科真菌DNA条形码的测试  

Can COI gene be used as DNA barcode for the nectriaceous fungi?

作　　者：赵鹏[1,2] 罗晶[1] 庄文颖[1] 

机构地区：[1]中国科学院微生物研究所真菌学国家重点实验室,北京100101 [2]鲁东大学菌物科学与技术研究院山东省食用菌技术重点实验室,山东烟台264025

出　　处：《菌物学报》2012年第2期243-250,共8页Mycosystema

基　　金：国家自然科学基金(No.31070015);中国科学院知识创新工程项目(No.KSCX2-EW-J-6)

摘　　要：以丛赤壳科33种76个菌株为材料,探讨COI基因作为该科DNA条形码的可能性。结果表明,该DNA片段存在较多内含子,为了获取某些种的短片段,需设计许多引物,PCR扩增与测序成功率低,难以达到便捷、快速的物种鉴定的目的。因此,COI不宜作为丛赤壳科的DNA条形码。对已获得的少数片段进行分析表明,该基因对丛赤壳科部分种具有较强的物种鉴别力。COI gene was tested with 76 strains of 33 species of the nectriaceous fungi to investigate its feasibility of serving as a DNA barcode.The results indicated that introns are present in some species,which made it difficult for PCR amplification and sequencing.COI gene is not a good DNA barcode for this group.Analysis of the obtained COI sequences showed that this gene may have the capacity to distinguish species of the nectriaceous fungi.

关 键 词：种内与种间差异 PCR扩增与测序成功率 邻接树 

分 类 号：Q943.2[生物学—植物学]