巴氏蘑菇多糖对铅中毒大鼠脾脏细胞因子mRNA表达的影响  

Effects of Agaricus blazei polysaccharides on the mRNA expression of cytokines in lead-poisoning rats

作　　者：程红艳[1,2] 常鼎然 冯翠萍[4,2] 常明昌[4,2] 王将[4] 李海杰[4] 李越[4] 

机构地区：[1]山西农业大学资源与环境学院,山西太谷030801 [2]山西省食用菌工程技术研究中心,山西太谷030801 [3]中国药科大学生命科学与技术学院,江苏南京210098 [4]山西农业大学食品科学与工程学院,山西太谷030801

出　　处：《菌物学报》2012年第2期258-266,共9页Mycosystema

基　　金：山西省自然科学基金(No.2010011043-1);山西农业大学博士科研启动经费项目(No.412564);山西省科技基础条件平台建设项目(No.2010091017);山西省科技攻关项目(No.20090311047)

摘　　要：为了研究巴氏蘑菇Agaricus blazei多糖对铅中毒大鼠脾脏细胞因子mRNA表达的影响,进而探索巴氏蘑菇多糖对铅中毒大鼠的免疫调节作用。选用45日龄SD大鼠,随机分为6组,每组8只,雌雄各半。分别为正常对照组、多糖组、铅中毒模型组、铅+50mg/kg·d多糖试验组、铅+100mg/kg·d多糖试验组、铅+200mg/kg·d多糖试验组。铅中毒模型组、铅+多糖试验组分别给予含0.2%醋酸铅饮水,自由饮用。饲养60d后,采集脾脏,荧光定量RT-PCR对其细胞因子IL-1β、IL-2、IL-6、TNF-α、IFN-γmRNA的相对表达量进行分析。结果表明,与正常对照组相比,IL-1β、IL-2、IL-6、TNF-α、IFN-γmRNA的表达量,多糖组升高,差异显著(P<0.05或P<0.01);铅中毒模型组下降差异极显著(P<0.01);试验组多糖浓度越高,表达量越高,铅+200mg/kg·d多糖组升高差异极显著(P<0.01)。与铅中毒模型组相比,试验组多糖浓度越高,表达量相对升高越多,铅+200mg/kg·d多糖组升高差异极显著(P<0.01)。说明巴氏蘑菇多糖对铅致大鼠免疫损伤具有保护作用。To investigate the immunoregulation activity of Agaricus blazei polysaccharides on lead-poisoning rats,the effects of polysaccharides on mRNA expression of cytokines in the spleen of lead-poisoning rats were studied.Forty-eight healthy 45 aged SD rats were randomly divided into six groups（female：male=1：1）,and named as control group,polysaccharide group,lead-poisoning model group,lead＋50mg/kg？d polysaccharide group,lead＋100mg/kg？d polysaccharide group and lead＋200mg/kg？d polysaccharide group respectively.The rats of lead-poisoning model group and lead＋polysaccharide group gave 0.2% lead acetate water for free drink.After treatment of 60 days,the spleens were collected,and the mRNA expressions of cytokines,such as IL-1β,IL-2,IL-6,TNF-α,IFN-γ were determined with fluorescent quantitative real-time polymerase chain reaction（FQRT-PCR）.The results showed that as compared with the control group,the mRNA expression levels of IL-1β,IL-2,IL-6,TNF-α,IFN-γ of polysaccharide group and lead＋200mg/kg？d polysaccharide group significantly increased at 0.05 or 0.01 level,and decreased significantly at 0.01 level in lead-poisoning model group.The mRNA expression levels of the tested cytokines in lead＋200mg/kg？d polysaccharide group increased at 0.01 level as compared with the lead-poisoning model group.The expression levels were incresed with the polysaccharides increased.The result suggested that Agaricus blazei polysaccharides played an improtant role in protecting the lead-induced immune system damage of animal.

关 键 词：荧光定量 白细胞介素 肿瘤坏死因子Α Γ干扰素 

分 类 号：R595.2[医药卫生—内科学] S646.1[医药卫生—临床医学]