抗创伤弧菌单克隆抗体杂交瘤细胞系的建立及特性鉴定  

Preparation and characterization of monoclonal antibody against Vibrio vulnificus

作　　者：付凯飞[1] 马骢[1] 郭建巍[1] 乔媛媛[1] 郝秀红[1] 王大鹏[1] 

机构地区：[1]海军总医院检验科,北京100037

出　　处：《细胞与分子免疫学杂志》2012年第3期279-281,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：全军医药卫生科研基金课题(06Z007)

摘　　要：目的:制备高效、特异的抗创伤弧菌的单克隆抗体(mAb),并进行特性鉴定。方法:用创伤弧菌菌体蛋白抗原免疫小鼠,利用杂交瘤细胞融合技术,制备抗创伤弧菌的杂交瘤细胞株。用ELISA法及Western blot等筛选、鉴定其与创伤弧菌溶血素蛋白(vvhA)及其他重要海洋细菌的交叉反应性和效价。结果:共获得5株抗创伤弧菌的mAb,鉴定结果表明,5株mAb均具有良好的特异性和免疫反应性。结论:获得5株抗创伤弧菌的特异性mAb,为建立创伤弧菌快速检测试剂盒提供了重要制剂。AIM： To prepare high-performance and specific monoclonal antibody（mAb） against Vibrio vulnificus and carry out characteriastion.METHODS： BALB/c mice were immunized with Vibrio vulnificus protein,and hybridoma cells against Vibrio vulnificus were produced by cellsion technique.The titers of mAbs against vvhA and cross-reaction between the anti-vvhA mAb and other other important marine bacteria were screened by ELISA and Western blot.RESULTS： Five strains of hybridoma were obtained.Identification result indicated that 5 mAbs have favourable specifity and immunoreactivity.CONCLUSION： Specific mAbs against Vibrio vulnificus were produced which provids an important preparation for establishing rapid-detection kit of detecting Vibrio vulnificus.

关 键 词：创伤弧菌 单克隆抗体 杂交瘤技术 

分 类 号：R392.11[医药卫生—免疫学]