白介素17A受体在系统性红斑狼疮患者外周血B淋巴细胞的表达及意义  

The expression and clinical significance of IL-17AR on peripheral blood B cells of patients with systemic lupus erythematosus

作　　者：王涛[1] 赵皓[2] 余晾[1] 陈琳洁[1] 范晓云[1] 谢长好[1] 梅永君[1] 李志军[1] 

机构地区：[1]蚌埠医学院第一附属医院风湿免疫科,安徽蚌埠233004 [2]蚌埠医学院第一附属医院中心实验室,安徽蚌埠233004

出　　处：《细胞与分子免疫学杂志》2012年第3期301-303,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家"十一五"科技支撑计划课题项目(2008BAl59B02);蚌埠医学院科研基金项目(BY0934)

摘　　要：目的:研究白介素17A受体(IL-17AR)在系统性红斑狼疮(SLE)患者外周血B淋巴细胞内的表达情况及其临床意义。方法:流式细胞术检测60例SLE患者及33例健康人外周血B细胞上IL-17AR的表达水平。并将其与有关临床及实验室指标进行相关性分析。结果:SLE患者组的IL-17AR+细胞表达比例为(47.58±17.20)%,高于正常对照组(40.71±11.82)%(P<0.05)。在SLE患者中:口腔溃疡组、有浆膜炎组、有肾脏病变组、有免疫异常组分别高于相应的无症状组(P<0.05)。在抗Sm-D1抗体阳性组及抗核小体抗体阳性组分别高于相应的抗体阴性组(P<0.05)。IL-17AR+细胞比例与SLE疾病活动指数(SLEDAI)、C反应蛋白(CRP)、甘油三酯呈正相关;与间接胆红素、血清白蛋白的表达负相关(P<0.05)。B淋巴细胞比例与血清IgG、ALT、直接胆红素的表达呈正相关;与胆固醇、甘油三酯、低密度脂蛋白的表达呈负相关(P<0.05)。结论:IL-17AR在SLE患者B细胞上表达上调,且与病情有一定的相关性。提示IL-17/IL-17AR可能在SLE发病中起着重要作用。AIM： To investgate the expressions of Interleukin-17A receptor（IL-17AR） on the peripheral blood B lymphocytes of SLE patients and to analyze the correlations between IL-17AR and clinical parameters.METHODS： Expression of IL-17AR on peripheral blood CD19 ＋ B lymphocytes were analyzed in 60 SLE patients and 33 healthy controls by flow cytometry.The difference of IL-17AR expression levels in two groups were compared.Furthermore,the correlation between IL-17AR expressions and clinical some measures,such as ESR,CRP,complement 3（C3）,complement 4（C4）,the amount of serum IgG,anti double strands DNA antibody,anti nuclear antibody,SLEDAI score and urine protein excretion were analyzed.RESULTS： Compared with healthy subjects,the proportions of B cells expressing IL-17AR were higher among SLE patients.In SLE patients,groups with mouth ulcer,serositis,renal lesions or immunologic abnormality were higher than the negative groups separately.The positive correlations were observed between IL-17AR expression levels and clinical measure of the SLEDAI,CRP and serum triglyceride level.The negative correlation was observed between IL-17AR expression levels and clinical measure of the serum indirect bilirubin level,serum albumin level.CONCLUSION： Interleukin-17A receptor expression increased on peripheral blood B cells of SLE patients,and correlate with clinical measures.

关 键 词：红斑狼疮 系统性 白介素17A受体 

分 类 号：R392.12[医药卫生—免疫学]