赛来昔布对瘢痕疙瘩成纤维细胞增殖及β-catenin和C-myc表达的影响  

The effect of Celecoxib on the expression of β-catenin and C-myc in human keloids fibroblasts

作　　者：宋凡伟 朱世泽[1] 王朝阳[1] 吴文艺[1] 王韶华[1] 李永峰[1] 杨维群[1] 

机构地区：[1]福建,泉州,福建医科大学第二临床医学院,362000

出　　处：《中国美容整形外科杂志》2012年第3期180-184,共5页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：福建省医学创新课题资助项目（2009-cx-21）;福建省教育厅科技项目基金资助（JA11321）

摘　　要：目的 探讨赛来昔布对体外培养的瘢痕疙瘩成纤维细胞增殖及β-catenin和C-myc表达的影响.方法 用不同浓度（0、25、50、100、125μmol/L）及不同作用时间（24、48、72h）的赛来昔布作用于人瘢痕疙瘩成纤维细胞,CCK-8（Cell Counting Kit-8）法检测人瘢痕疙瘩成纤维细胞的增殖活性,QRT-PCR、Western-blot检测体外培养的人正常皮肤及瘢痕疙瘩成纤维细胞中β-catenin和C-myc 的mRNA及蛋白表达.结果 CCK-8法显示,赛来昔布可显著抑制体外培养的人瘢痕疙瘩成纤维细胞增殖（P ＜0.05）,呈剂量和时间依赖性;QRT-PCR和Western-blot显示：①与体外培养的人正常皮肤成纤维细胞相比,体外培养的人瘢痕疙瘩成纤维细胞中,C-myc 及β-catenin蛋白的表达明显上调（P ＜0.05）,而β-catenin mRNA表达未见明显增加;②不同浓度（0、25、50、100、125μmol/L）赛来昔布作用的体外培养的人瘢痕疙瘩成纤维细胞中,随着赛来昔布浓度增高,β-catenin和C-myc的表达逐渐降低,各组之间差别明显（P ＜0.05）.结论 人瘢痕疙瘩的发生与Wnt信号通路异常密切相关;赛来昔布能通过Wnt信号通路抑制瘢痕疙瘩成纤维细胞增殖,有望用于瘢痕疙瘩的临床治疗.Objective To explore the effect of Celeeoxib on proliferation of human keloids fibroblasts and the expression of -catenin and C-myc. Methods Different concentrations（0, 25, 50, 100, 125 μmol/ L） and effect time（24, 48, 72 hours） of Celecoxib acted on keloid fibroblasts, and CCK28 assay was adopted to evaluate cell survival, QRT-PCR and Western blot were performed to detect the expression of mRNA and protein of β-catenin and C-myc of the normal skin and keloid fibroblasts in vitro. Results CCK-8 assay exibitted that Celecoxib could significantly inhibit the proliferation and activity of human keloid fibroblasts with different concentrations and effect time. QRT-PCR and Western blot showed that the protein of C-myc and ^-catenin were more significantly upregulated in the keloids fibroblasts than that in the normal skin, and mRNA of β-cate- nin had no change; Celeeoxib with different concentrations acted an human keloids fibroblasts in vitro, with the elevation of the concentration, the expression of the C-myc and β-eatenin droped, there were significant differ- ences between these groups （ P 〈 0.05 ）. Conclusion Wnt signaling pathway abnormalities is an important mechanism for the occurrence of human keloid. Celeeoxib can inhibit the proliferation of human keloids fibro- blasts correlat with its effect on Wnt signaling pathway

关 键 词：瘢痕疙瘩 赛来昔布 成纤维细胞 细胞增殖 Β-CATENIN C-MYC 

分 类 号：R619.6[医药卫生—外科学]