力生长因子E肽与应力刺激对成骨细胞基因表达的影响  

Effect of MGF-Ct24E and mechanical stimulation on gene expression of osteoblasts

作　　者：林福春[1,2] 张兵兵[1,2] 辛娟[1,2] 向燕[1,2] 鲜成玉[1,2] 邓墨渊[1,2] 彭琴[1,2] 王远亮[1,2] 

机构地区：[1]重庆大学生物流变科学与技术教育部重点实验室,重庆400030 [2]重庆大学生物工程学院国家"985工程"生物材料与仿生工程研究中心,重庆400030

出　　处：《医用生物力学》2012年第1期65-71,共7页Journal of Medical Biomechanics

基　　金：国家自然科学基金资助项目(11032012;30870609);重庆市自然科学基金资助项目(2009BB4382;2010BB5225)

摘　　要：目的应用基因芯片技术分析在力生长因子E肽(MGF-Ct24E)和应力作用下成骨细胞基因表达的差异。方法体外培养原代成骨细胞,分别对细胞施加周期性拉伸刺激(应变12%,频率0.5 Hz)和MGF-Ct24E(50 mg/L)直接作用,基因芯片技术分析力学刺激和MGF-Ct24E作用对原代成骨细胞基因表达谱的影响,并用定量PCR验证基因芯片实验结果。结果与对照组相比,力学加载组共发现差异表达基因1 866个,其中上调基因1 113个,下调基因753个;MGF-Ct24E处理组共发现差异表达基因1 178个,其中上调基因796个,下调基因382个。GO分析发现两者的差异基因表达谱具有一致性,并且差异表达基因主要涉及细胞增殖与分化调节、细胞对应力刺激的响应和力学转导通路等。定量PCR实验结果验证的差异表达基因与芯片实验结果一致。结论基因表达谱分析显示应力刺激和MGF-Ct24E作用对成骨细胞的基因表达具有类似的调控效应,为后续使用MGF-Ct24E治疗骨修复以弥补应力刺激不足的研究提供了新的思路。Objective To identify the differentially expressed genes of osteoblasts under the stimulation of mechano growth factor E peptide（MGF-Ct24E） and mechanical stress by microarray analysis.Methods Primary osteoblasts were cultured in vitro,which were subjected to mechanical stimulation（with the mechanical strain of 12% and frequency of 0.5 Hz） and MGF-Ct24E treatment（50 mg/L）,respectively.The gene expression profiles were analysed by cDNA microarrys and quantitative PCR was used to validate the microarray data.Results Compared with the control group,1 866 genes were found to have differentially expressed in the mechanical loading group,in which 1 113 genes were up-regulated,while 753 genes were down-regulated.1 178 genes were found to have differentially expressed in the MGF-Ct24E group,in which 796 genes were up-regulated and 382 genes were down-regulated.GO analysis suggested that the gene expression profile of MGF-Ct24E group was consistent with that of the mechanical loading group and differentially expressed genes were mainly involved in cell proliferation and differentiation,response to mechanical stress and mechaotransduction.Conclusions The microarray analysis showed that MGF-Ct24E treatment had similar effects with the mechanical loading on the gene expression of osteoblasts,which might provide a novel approach to study the usage of MGF-Ct24E for treating bone repair in the absence of mechanical stimulation.

关 键 词：成骨细胞 力生长因子 应力刺激 基因芯片 基因表达 细胞培养 

分 类 号：Q291[生物学—细胞生物学] R68[医药卫生—骨科学]