基于mtD NACOⅠ基因序列的云南榕母管蓟马不同地理种群的遗传分化分析  

Analysis of genetic differentiation among geographic populations of Gynaikothrips ficorum (Marchal) (Thysanoptera:Phlaeothripidae) in Yunnan,southwestern China,based on mtDNA COⅠ gene sequences

作　　者：张利娟[1,2] 沈登荣[3] 孙跃先[1,2] 李正跃[1,2] 张宏瑞[1,2] 

机构地区：[1]云南农业大学农业生物多样性与病虫害控制教育部重点实验室,昆明650201 [2]云南农业大学植物保护学院,昆明650201 [3]红河学院农学系,云南蒙自661100

出　　处：《昆虫学报》2012年第2期199-207,共9页Acta Entomologica Sinica

基　　金：云南农业大学农业外来入侵生物可持续控制省创新团队项目(2011HC005);云南省高校科技创新团队支持计划项目;农业部公益性行业科研专项项目(201103026;200803025);云南省自然基金面上项目(2007C054M)

摘　　要：榕母管蓟马Gynaikothrips ficorum(Marchal)是一种已扩散至各大洲的榕树主要害虫,目前在云南省热带及亚热带区域发生危害亦较为严重。为了揭示榕母管蓟马在云南省不同地理种群间的遗传变异,测定了10个地理种群145个个体的mtDNACOⅠ基因的646bp序列,对地理种群间的序列变异和遗传分化进行了分析。结果表明:10个地理种群间的COⅠ基因共有38个变异位点和6个单倍型,其中1个单倍型为8个种群所共享。种群间的遗传距离范围为0～0.043,其中瑞丽、芒市、玉溪、呈贡种群间的遗传距离最小,宜良和陇川、墨江种群间的遗传距离最大,种群遗传距离大小与其相对地理距离的远近之间没有相关性。分子方差分析显示3组(组1:陇川、瑞丽、芒市、玉溪、呈贡、墨江、临沧、勐腊8个种群;组2:蒙自种群;组3:宜良种群)之间已经具有明显的遗传分化,Fst值为0.9828(P<0.05),Nm值为0.01,但是仅0.0172的遗传变异来自组内。采用邻接法(NJ)构建分子系统树,单倍型被分成3组与各自的地理区域相对应的簇群,各组之间未发现共享的单倍型。分子系统树显示3组的聚类结果与地理分布格局并不对应。综合采集地寄主植物的状况,初步推测蒙自和宜良种群出现的遗传分化可能是由于寄主植物生长状况及品种不同引起的。各地理种群中的单倍型在网络中介图上散布在不同的分布群中,缺乏明显的地理分布格局。Gynaikothrips ficorum （Marchal） is a major insect pest damaging Ficus microcarpa and has expanded to all the continents. At present, it damages F. microcarpa seriously in tropical and subtropical areas in Yunnan province. In order to reveal the genetic differentiation among geographic populations of G. ficorum, a 646 bp segment of the mitochondrial cytochrome c oxidase subunit Ⅰ （COⅠ） gene was sequenced from 145 individuals among 10 geographic populations in Yunnan province, and the sequence variability of the COⅠ gene and genetic differentiation among them were analyzed. The results showed that 38 variable sites were detected and six haplotypes were defined based on the COⅠ sequences among 10 geographic populations of G. ficorum, including one haplotype shared by eight populations. The genetic distances ranged from 0 （among populations of Ruili, Mangshi, Yuxi and Chenggong） to 0.043 （between Yiliang population and populations of Longchuan and Mojiang） among the 10 geographic populations, indicating that the genetic distances among G. ficorum populations were not associated with their geographic distances. Analysis of molecular variance showed that 10 geographic populations were clustered into three groups （group I： Longchuan, Ruili, Mangshi, Yuxi, Chenggong, Mojiang, Lincang, and Mengla; group Ⅱ： Mengzi; group Ⅲ： Yiliang）. The Fst value was 0.9828 （P0.05）, and the Nm value was 0.01, indicating apparent genetic differentiation among the three groups, but there was only 0.0172 genetic variation from intragroup. A molecular phylogenic tree constructed using neighbor-joining （NJ） method showed that the six haplotypes were clustered into three clades associated with geographic regions. No shared haplotypes were found among the three groups. Molecular analysis showed that these three major groups/clades did not coincidence with the geographic distribution. Based on the information of host plants, we preliminarily presumed that the genetic differentiation between the two po

关 键 词：榕母管蓟马 地理种群 遗传距离 遗传分化 COⅠ基因 

分 类 号：Q968[生物学—昆虫学]