体外培养人表皮黑素细胞样本制备及超微结构观察  

Ultrastructural observations and preparation of cultured human epidermal melanocytes'samples

作　　者：徐丹丹[1] 李伟秋[2] 方斌[1] 黄清霞[3] 梁伟全[3] 梁彦[3] 钟耀棠[3] 王腾彬[3] 周远军[3] 陈玮莹[1] 

机构地区：[1]汕头大学医学院生物化学教研室,汕头515041 [2]汕头大学医学院电镜室,汕头515041 [3]汕头大学医学院2007级7年制临床医学专业,515041

出　　处：《临床与实验病理学杂志》2012年第3期295-297,共3页Chinese Journal of Clinical and Experimental Pathology

基　　金：广东省自然科学基金(9151806001000009);广东省大学生创新实验(汕头大学医学院杰出大学生科研基金)(20105709)

摘　　要：目的探讨体外培养正常人表皮黑素细胞(melanocytes,MC)透射电镜制样方法及超微结构观察的作用。方法采用M254 MC培养基及添加剂HMGS培养和纯化人表皮MC;分别采用常规制样方法和以PET聚酯薄膜为培养细胞的支持物制备透射电镜样本。结果人表皮MC透射电镜观察发现,常规制样标本可观察人表皮MC及其树状突起横切面的超微结构。PET聚酯薄膜作为培养MC支持物的样本可观察人表皮MC和树状突起的完整超微结构及树状突起的纵切面超微结构。结论 PET聚酯薄膜可作为人表皮MC体外培养良好的支持材料。应用不同样本制备方法可以观察MC的超微结构,使用PET聚酯薄膜作为细胞支持物制备的标本更利于MC黑素体代谢和转移的观察。Purpose To investigate the preparation methods of TEM samples for cultured human epidermal melanocytes and to observe the ultrastructures of the cells.Methods Melanocytes were isolated from children＇s foreskins and cultured in M254 medium that was supplemented with human melanocyte growth supplement.Purified melanocytes were harvested from culture flask and TEM sample was prepared by conventional method,or cultured on PET film and allowed to attach and then TEM sample was prepared,respectively.Results Transmission electron microscopy showed that the ultrastructure of melanocytes and dendrites in transverse section were observed in samples prepared with conventional method.The intact ultrastructure of melanocytes and their dendrites could be observed in longitudinal section while PET film was applied as cellular support.Conclusions The PET film is an excellent cellular supporting material for the cultivation of human epidermal melanocytes.The ultrastructures of human epidermal melanocytes could be observed in samples prepared in different ways.But the sample that using PET film as cellular support is much beneficial for the ultrastructural observation of metabolism and transferring of melanosome in melanocytes.

关 键 词：黑素细胞 细胞培养 黑素体 PET聚酯薄膜 透射电镜 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] Q248[医药卫生—基础医学]