^(60)Coγ射线照射大鼠涎腺组织后Mrell蛋白表达的初步研究  

The expression of Mrell Protein in salivary glands of rat after ^(60)Coγ irradiation

作　　者：沈洁[1] 王代友[1] 曹阳[1] 林丹[1] 陈超梅[1] 

机构地区：[1]广西医科大学附属口腔医院口腔颌面外科,广西南宁530021

出　　处：《临床口腔医学杂志》2012年第3期144-149,共6页Journal of Clinical Stomatology

基　　金：国家自然科学基金资助(30960420)

摘　　要：目的:检测Mre11基因及其蛋白在放疗后的大鼠腮腺和颌下腺组织中的表达变化。方法:选取近交系雄性Wistar大鼠60只,按单次照射0 Gy,3 Gy、6 Gy、9 Gy、12 Gy、15 Gy剂量分成6组,用60Coγ射线照射大鼠头颈部,2h后收集大鼠两侧腮腺和颌下腺组织。用透射电镜观察涎腺上皮细胞超微结构变化;用RT-PCR检测Mre11的基因表达情况;用免疫组化检测Mre11蛋白表达情况。结果:透射电镜显示:放射后大鼠腮腺腺泡细胞胞质和胞膜均出现不同程度的损坏,随着剂量的增加,这种损坏逐渐加剧,未见到再生、恢复的变化。颌下腺和导管变化不明显。RT-PCR检测Mre11mRNA表达无统计学差异。免疫组化检测Mre11蛋白表达有统计学差异。结论:治疗剂量的60Coγ照射对正常涎腺组织呈不可逆的损伤,损伤的程度具有剂量依赖性;大鼠涎腺细胞Mre11mRNA的表达无剂量依赖性;大鼠涎腺细胞Mre11蛋白的表达与辐射剂量以及组织类型有关,随剂量的增加先增强后减弱。Objective： To investigate the relationship between Mrel 1 protein expression and radiation exposure doses in irradiated salivary glands of rats. Method： Sixty male Wistar rats were chosed in our study, Then made the salivary glands due to exposure to ionizing radiation, each group received a dose of 0 Gy, 3 Gy, 6 Gy,9 Gy, 12 Gy, 15 Gy respectively.after 2 hours, collect the salivary glands. Through transmission electron microscope to examine the effect of ionizing radiation on rat parotid gland.Expression level of Mrel 1 mRNA was detected through RT-PCR. Mrel 1 protein expression was assayed with immunocytochemistry. Result：with the increse of dose,the irradiated rats had been destroyed. The damage became more and more serious. And there was no recovery.the one-way analysis of variance shows that the expression of Mrell mRNA had not statistically significant between each group. But the expression of Mrel 1 protein had statistically significant. Conclusion： ^60Coγirradiation can lead damage to salivary glands, and the level of damage had much to do with radiation ex- posure dose, and the damage is inreversible, the expression of Mrel 1 mRNA was not dependented on radiation exposure dose,while the expression of Mrell protein depend on the doses and the tissues. And it is enhanced first and then became weakened.

关 键 词：Mre11蛋白 大鼠涎腺细胞 放射敏感性 DNA双链断裂 

分 类 号：R816.98[医药卫生—放射医学] Q786[医药卫生—临床医学]