2006年至2008年上海市住院腹泻患儿A组轮状病毒的分子流行病学调查  

Molecular epidemiology study on group A Rotavirus in hospitalized children with diarrhea in Shanghaiduring 2006--2008

作　　者：卢丽娟[1] 徐锦[1] 钟华清[1] 苏犁云[1] 曹凌峰[1] 徐梦华[1] 

机构地区：[1]复旦大学附属儿科医院儿科研究所, 上海,201102

出　　处：《中华传染病杂志》2012年第2期90-94,共5页Chinese Journal of Infectious Diseases

摘　　要：目的分析上海地区住院腹泻患儿A组轮状病毒（RV）基因型别、流行季节及年龄分布，以了解本地区A组RV的分子流行病学动态变化特征，为婴幼儿腹泻的预防和控制提供理论依据。方法2006年1月至2008年12月共收集来自复旦大学附属儿科医院5岁以下腹泻患儿的5176份粪便标本。抽取其中RV抗原阳性标本380份，采用套式多重RT-PCR法进行RV的基因分型。结果2006-2008年RV腹泻主要的流行季节是10-12月份，〈3岁的患儿占全部患儿的96．8％。380份粪标本中，222份为G3型，占58．4％，41份为G9型，占10．8％。3年间GI型、G2型均少见，G混合型以G3和G9型的混合为主。2006年和2008年均以P[8]型的流行为主，分别占64．6％（53／82）和46．8％（58／124）。2007年以P[4]型为主，占38．5％（67／174）。P[6]和P[9]型所占比例较低，P混合型以P[8]和P[4]型的混合为主。G未分型和P未分型在3年间均呈上升趋势。PE8]G3型（20．5％）是上海地区2006-2008年间最主要的A组RV流行株，其次包括PE4]G3型及P[m]G3型。结论2006-2008年上海地区A组RV显示了新的分子流行病学变化特征，包括主要流行型别的改变，流行型别更趋于多样化。Objective To investigate the dynamic characteristic of molecular epidemiology of group A Rotavirus （RV） by analyzing viral genotypes, disease seasonality, and the patientsp age distribution, so that to provide theoretical basis for prevention and control of RV diarrhea in children. Methods A total of 380 RV antigen positive samples were selected from 5176 stool specimens collected from 〈5 year-old patients with acute diarrhea who were admitted to ChildrenJs Hospital of Fudan University during January 2006 to December 2008. Multiplex nested reverse transcriptionpolymerase chain reaction （RT-PCR） was used to analyze the RV genotypes. Results During 2006-- 2008, the incidence of RV related diarrhea peaked from October to December and about 96.8% of all RV episodes occurred in patients younger than 3 years old. The predominant genotype was G3 which accounted for 58.4% （222/380）, G9 was an emerging genotype with the prevalence rate as high as 10.8% （41/380）. G1 and G2 types were rarely found during the three years. Infections with both G3 and G9 were the major mixed genotype G infection. Genotype P [8] was predominant with the prevalence rates of 64.6% （53/82） and 46.8% （58/124） in 2006 and 2008, respectively, whereasFudan University during January 2006 to December 2008. Multiplex nested reverse transcription polymerase chain reaction （RT-PCR） was used to analyze the RV genotypes. Results During 2006- 2008, the incidence of RV related diarrhea peaked from October to December and about 96.8%of all RV episodes occurred in patients younger than 3 years old. The predominant genotype was G3 which accounted for 58.4% （222/380）, G9 was an emerging genotype with the prevalence rate as high as 10.8% （41/380）. G1 and G2 types were rarely found during the three years. Infections with both G3 and G9 were the major mixed genotype G infection. Genotype P[8] was predominant with the prevalence rates of 64.6% （53/82） and 46.8% （58/124） in 2006 and 2008, respectively, whereasgenotype P[4]

关 键 词：腹泻 轮状病毒属 基因型 儿童 流行病学 分子 

分 类 号：R72[医药卫生—儿科]