胰泌素刺激-磁共振胰胆管成像定量分析猫慢性胰腺炎的胰腺外分泌功能  

Quantitative analysis of pancreatic exocrine function of chronic pancreatitis in cat models with secretin-stimulated magnetic resonance cholangiopancreatography

作　　者：张霆霆[1] 王莉[2] 李玉华[1] 黄志军 陆建平[2] 

机构地区：[1]上海交通大学医学院附属新华医院放射科,上海200092 [2]第二军医大学附属长海医院影像医学科,上海200433 [3]盐城市第一人民医院普外科,江苏盐城224005

出　　处：《中国医学影像技术》2012年第3期416-420,共5页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金(81070371)

摘　　要：目的探讨胰泌素刺激-磁共振胰胆管成像(S-MRCP)定量评估猫慢性胰腺炎(CP)胰腺外分泌功能的价值。方法将32只健康成年猫分为实验组(n=24)及对照组(n=8);实验组剖腹行胰管不全结扎,建立CP模型,对照组仅行无菌开腹,不结扎胰管。对实验组分别于建模后3、5、7周分批行S-MRCP检查,每批次8只;对照组于缝合关腹后立即进行检查。S-MRCP检查完成后对动物胰腺进行病理学分析,根据病理结果将实验组分为3个亚组:CP-Ⅰ(轻度CP)、CP-Ⅱ(中度)、CP-Ⅲ(重度CP)。对各组注射胰泌素前、后胃肠道液体量的增加程度(FVID)进行统计学分析。结果实验组24只中,15只建模成功,CP-Ⅰ、CP-Ⅱ、CP-Ⅲ亚组分别为7、5、3只。注射胰泌素5min及15min后实验组整体FVID低于对照组(F=9.742、13.543,P均<0.05)。对照组与CP-Ⅱ亚组间、对照组与CP-Ⅲ亚组间及CP-Ⅰ与CP-Ⅲ亚组间差异均有统计学意义(P均<0.05);其余各组间差异虽无统计学意义(P均>0.05),但随着胰腺炎程度加重,FVID有逐渐下降趋势。结论利用S–MRCP可通过测量注射胰泌素前、后胃肠道液体量的变化评价猫CP模型胰腺外分泌功能。Objective To assess the value of secretin-stimulated magnetic resonance cholangiopancreatography（S-MRCP） in quantitative analysis of pancreatic exocrine function in chronic pancreatitis（CP） cat models.Methods Thirty-two healthy adult cats were divided into experimental group（n=24） and control group（n=8）.Incomplete ligation of pancreatic duct was performed on cats in experimental group in order to establish CP models,while cats in control group received a sham operation.S-MRCP was performed in 3,5,and 7 weeks after ligation with 8 cats examined at each time point in experimental group.Cats in control group underwent postoperative S-MRCP immediately.After S-MRCP,cats in experimental group were classified into three subgroups according to the histological analysis of the pancreas： CP-Ⅰ（slight）,CP-Ⅱ（moderate） and CP-Ⅲ（severe）.Increasing degrees of fluid volume（FVID） in gastrointestinal tract before and after injecting secretin were statistically analyzed.Results Fifteen CP models were established successfully in experimental group（n=24）,including 7 in CP-Ⅰ,5 in CP-Ⅱ and 3 in CP-Ⅲ subgroups.FVID of experimental group was lower than that of control group at 5 and 15 minutes after secretin injection（F=9.742,13.543,both P〈0.05）.There were significant differences of FVID between control group and CP-Ⅱ subgroup,as well as between control group and CP-Ⅲ subgroup（both P〈0.05）.Moreover,significant difference of FVID was found between CP-Ⅰ and CP-Ⅲ subgroup（P〈0.05）.Although there was no significant difference between other groups（all P〉0.05）,FVID showed a downward trend with increased worsening degrees of CP.Conclusion S-MRCP can be used to quantify pancreatic exocrine function of cat CP models by detecting FVID changes before and after secretin injection

关 键 词：胰腺炎 慢性 胰腺外分泌功能 胰泌素 胰胆管造影术 磁共振 猫 

分 类 号：R445.2[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]