小鼠对仙台病毒Tianjin株易感性研究  

Study of the Susceptibility of Mice to Sendai Strain Tianjin

作　　者：赵桂金[1] 李梅[1] 

机构地区：[1]天津医科大学基础医学院微生物学教研室,天津300070

出　　处：《病毒学报》2012年第2期103-107,共5页Chinese Journal of Virology

摘　　要：研究129Sv、DBA/2、Kunming、BALB/c四种小鼠对仙台病毒Tianjin株的感染特点,并通过观察易感性的不同,确定适于研究此病毒致病性及疫苗的小型啮齿类实验动物。用9～11d龄鸡胚接种仙台病毒Tianjin株,72h后收集尿囊腔内效价为1:1 280病毒液,用5μl和6倍稀释的30μl病毒液分别接种129Sv、DBA/2、Kunming、BALB/c小鼠,观查12d小鼠体重变化,计算生存率。用6倍稀释的30μl病毒液接种Kunming、BALB/c小鼠,于接种前第1天以及接种后第4、7天断颈处死,取左肺制成切片,HE染色观察病理改变,综合判断仙台病毒Tianjin株对四种鼠感染的易感性的不同。129Sv、DBA/2小鼠在接种仙台病毒Tianjin株5μl后,最高平均体重下降分别为13.0%、4.7%,四种鼠12d生存率均为100%;接种稀释的30μl病毒液,129Sv、DBA/2、Kunming、BALB/c最高平均体重下降21.7%、30.3%、16.7%、9.6%;12d生存率分别为20%、0%、80%、100%。Kunming鼠在感染后第4、7d的肺组织病理改变较BALB/c严重,表现为大量炎细胞渗出,粘膜下层实质性增厚。以上实验结果表明DBA/2对仙台病毒Tianjin株感染最易感,BALB/c耐受性最强,易感顺序为DBA/2>129Sv>Kunming>BALB/c。DBA/2和129Sv小鼠可作为仙台病毒Tianjin株致病性及疫苗研究的首选实验动物。To explore the infectivity characteristics and susceptibility of Sendai strain Tianjin in 129Sv,DBA/2,Kunming and BALB/c mice and determine the optimal small rodent animal model for strain Tianjin research,the Sendai strain Tianjin was propagated for 72h in 9-11 day-old chicken embryos,the allantoic fluids were then harvested and the virus titer（1：1280） was detected by hemagglutination assay.Four different kinds of mice were intranasally inoculated with 5μl and the diluted 30μl virus solution.The weight loss of mice was monitored for 12 consecutive days and the survival rate was observed.Kunming and BALB/c mice were sacrificed on the first day prior to infection and on the fourth and seventh days post infection of the diluted 30μl Sendai strain Tianjin.Their left lobes of lung were fixed with 4% formalin for histopathologic examination.The maximum percentage of average weight loss of 129Sv,DBA/2 were 13.0%,4.7% with 100% survival rate when 129Sv,DBA/2,Kunming and BALB/c were inoculated with 5μl virus solution,while the mice were inoculated with diluted 30μl virus solution,the maximum percentage of average weight loss reached 21.7%,30.3%,16.7% and 9.7% with the survival rate of 20%,0%,80% and 100%.Lung infections of mice Kunming on the fourth and seventh day post infection were more severe than that of BALB/c,showing a large number of inflammatory cell exudation and thickening of the submucosa.It suggested that DBA/2 was the most susceptible to the infection of strain Tianjin.The mice susceptibility ranked as DBA/2〉129Sv〉Kunming〉BALB/c.Mice DBA/2 and 129Sv could be used as the optimal small rodent animal models in the research of pathogenicity and vaccine of Sendai strain Tianjin.

关 键 词：仙台病毒 Tianjin株 体重下降 生存率 易感性 

分 类 号：S852.65[农业科学—基础兽医学]