2009年云南省2株肠道病毒71型分离株全基因组序列遗传特性分析  

Complete Genome Sequence Characteristics of Human Enterovirus 71 Strain Isolated in Yunnan,China

作　　者：李华[1,2] 邵聪文[1,2] 潘玥[1,2] 柯华昕[1,2] 马绍辉[1,2] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所,昆明650118 [2]云南省重大传染病疫苗研发重点实验室,昆明650118

出　　处：《病毒学报》2012年第2期108-113,共6页Chinese Journal of Virology

摘　　要：对2009年云南省肠道病毒71型分离株KMM09和KM186-09进行全基因组序列测序,并与我国及其它国家流行的EV71基因型进行比较和进化分析。KMM09和KM186-09基因组长为7409bp,编码2193个氨基酸,VP1系统进化分析显示2009年云南分离株属于C4基因型的C4a亚型。在结构区,与其它基因型相比较,C基因型之间的核苷酸和氨基酸的同源性高于其它基因型;而在非结构区,C4与B基因型和CA16原型株G10同源性高于其它C基因亚型。通过RDP3重组软件和blast比对分析,发现EV71C4基因型与B3基因型,与CA16原型株G10的基因组在非结构区存在重组。EV71全基因组序列的比较和分析,对了解引起我国手足口病暴发或流行C4基因亚型EV71毒株的遗传特性具有重要意义。The complete nucleotide sequence of two human enterovirus 71 strains（KMM09 and KM186-09） isolated in Yunnan,China,were determined by RT-PCR and sequencing.As with other human enteroviruses,the genomes were 7 409 nucleotides（nts） in length and encoded 2 193 aa.Phylogenetic analysis based on VP1 regions revealed that the two isolates belonged to subgenotype C4a.In structural genomic regions,subgenotype C4 was most homologous to other strains of C genotype when compared to other genotypes.In non-structural genomic regions,subgenotype C4 was more homologous to CA16/G10 and other strains of B genotype than to other strains of C genotype.RDP3 and Blast analysis displayed evidence of recombination in non-structural genomic regions between subgenotype B3 and C4,C4 and CA16/G10.The full-length genome of the human enterovirus 71 strains provided an overview of the diversity of genetic characteristics of a circulating human enterovirus 71.

关 键 词：肠道病毒71型 全基因组 遗传特性 

分 类 号：R373.2[医药卫生—病原生物学]