中国大陆2010年非野毒Ⅱ型脊髓灰质炎病毒基因特征分析  

Analysis of Genetic Characteristics of Type Ⅱ Non-wild Poliovirus in China's Mainland,2010

作　　者：蒋华芳[1,2] 严冬梅[2] 祝双利[2] 王东艳[2] 张勇[2] 朱晖[2] 安洪秋[2] 许文波[2] 孔晓慧[1] 

机构地区：[1]首都医科大学附属北京儿童医院,北京100045 [2]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《病毒学报》2012年第2期130-135,共6页Chinese Journal of Virology

基　　金：传染病及病媒生物控制适宜技术研究-维持无脊灰时期外环境脊髓灰质炎病毒检测技术的建立及推广应用(2008BA156B02);(艾滋病和病毒性肝炎等重大传染病防治科技重大专项)病原体网络化监测技术研究-麻疹和脊灰监测技术(2008ZX10004-008);(艾滋病和病毒性肝炎等重大传染病防治科技重大专项)自然疫源性传染病病原谱流行规律及变异研究(2012ZX10004201)

摘　　要：为维持中国无脊髓灰质炎(脊灰)状态及疫苗免疫策略的转变提供科学依据,本文对中国(不包括港澳台地区,以下同)2010年从急性弛缓性麻痹(Acute flaccid paralysis,AFP)病例监测系统分离的123株非野毒Ⅱ型脊灰病毒(Po-liovirus,PV)进行基因特征分析。利用逆转录-聚合酶链反应(Reverse transcription-polymerase chain reaction,RT-PCR)扩增VP1编码区,并对扩增产物进行序列测定,测序结果经Sequencher 4.8、BioEdit 7.0.9和MEGA 5.0软件分析。结果显示2010年未发现脊灰野病毒(Wild poliovirus,WPV)。65株PV在VP1编码区nt2 909发生突变,同时该位点又是已知的神经毒力决定位点。从云南省分离到2株疫苗衍生脊灰病毒(Vaccine-derived poliovirus,VDPVs),经流行病学和实验室检测结果分析表明,这2株都不同于以往发现的VDPVs,是新发现的VDPVs。建议加强全国AFP病例的流行病学和实验室监测和检测,为及时发现VDPVs传播和WPV的输入性暴发提供科学依据。To study the genetic characteristics of 123 type Ⅱ non-wild polioviruses isolated from acute flaccid paralysis（AFP） cases in China's Mainland in 2010,provide the scientific basis for maintaining the ＂polio-free＂status,and the switching use of polio vaccine for China.VP1 gene was amplified by reverse transcription-polymerase chain reaction（RT-PCR） and the PCR products were then sequenced.The sequence results were analyzed with Sequencher 4.8,BioEdit 7.0.9 and MEGA 5.0.Of 65 strains,nt2909 was found to be a mutation hotspot,and also a neurovirulence determinant in VP1 region.During 2010,two vaccine-derived polioviruses（VDPVs）were isolated from Yunnan province,China and no wild poliovirus（WPV） was isolated.The epidemiological studies and laboratory results of the two VDPVs showed that they were newly discovered VDPVs because of the genetic difference from other VDPVs strains isolated in the world,implying the sensitive poliovirus surveillance network could timely detect the transmission of VDPVs and the importation of WPV.

关 键 词：Ⅱ型脊髓灰质炎病毒 VP1编码区 基因特征 

分 类 号：R373.2[医药卫生—病原生物学]