α-硫辛酸对在H9c2细胞在H_2O_2导致的氧化应激损伤中的保护作用  

Protection of α-Lipoic Acid Against H_2O_2-induced Oxidative Stress in H9c2 Cells

作　　者：刘羿男[1] 王卫平[2] 贾竹青[2] 陈苹[2] 马康涛[2] 周春燕[2] 

机构地区：[1]北京大学医学部基础医学院细胞生物学系,北京100191 [2]北京大学医学部基础医学院生物化学与分子生物学系,北京100191

出　　处：《中国生物化学与分子生物学报》2012年第3期227-233,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.81071675;No.81170713;No.81160103)~~

摘　　要：缺血再灌注产生的氧自由基会导致心肌细胞凋亡.近年研究发现,α-硫辛酸(α-lipoic acid,LA)具有抗氧化作用,但LA是否能够对抗心肌细胞凋亡,保护心脏功能的作用尚未明确.本研究利用H2O2诱导的心肌细胞H9c2氧化应激模型,分别用CCK-8方法检测细胞存活率、Hoechst33342染色观察细胞核的形态变化、流式细胞术检测细胞凋亡率、real-time PCR法检测Bcl-2/Bax基因表达变化,评价LA是否具有对抗氧化损伤引起的心肌细胞凋亡能力.结果显示,LA能提高H2O2损伤的H9c2细胞存活率,降低心肌细胞凋亡,而且LA通过上调Bcl-2的表达而发挥抑制细胞凋亡的作用.研究结果证实,LA对氧化应激损伤的心肌细胞具有较好的保护作用.该研究为LA在临床上用于治疗氧化应激引起的心肌细胞凋亡提供了实验依据.The oxygen free radicals produced by cardiac ischemia/reperfusion injury can cause cardiomyocyte apoptosis.α-Lipoic acid（LA） plays a role in anti-oxidation.However,whether LA has cytoprotective potential to protect cardiomyocytes from apoptosis remains unclear.In this study,H2O2-induced injury in H9c2 cells was used as a cell model,the anti-apoptosis potential of LA against oxidative injury was evaluated by CCK-8 determination for cell viability,by Hoechst33342 staining for nucleus morphology,by flow cytometry assay for apoptosis rate,by real-time PCR assessment for anti-apoptosis Bcl-2 and pro-apoptosis Bax gene expression.We found that LA could increase the cell survival of H2O2-induced injury in H9c2 cells and prevent H9c2 cells from oxidative stress-induced apoptosis.Furthermore,LA mediated the anti-apoptosis role by upregulating Bcl-2 expression.The results suggest that LA may have protective effect for the H2O2-induced apoptosis in H9c2 cells.These findings will contribute to the treatment of cardiomyocyte apoptosis induced by oxidative stress.

关 键 词：Α-硫辛酸 过氧化氢 氧化应激 H9C2细胞 凋亡 

分 类 号：R33[医药卫生—人体生理学]