D-半乳糖致衰老小鼠与幼龄小鼠的垂体差异蛋白质分析  

Differential Proteomic Analysis of Pituitary Gland from D-galactose Induced Aging Mice

作　　者：曹锐[1] 银兴峰 李江林[1] 谢纯良[1] 王迎[1] 王贤纯[1] 陈平[1] 梁宋平[1] 

机构地区：[1]湖南师范大学生命科学学院,长沙410081

出　　处：《中国生物化学与分子生物学报》2012年第3期248-253,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.81070353;No.30770437;No.31000375)~~

摘　　要：脑垂体是控制动物生长发育衰老死亡非常重要的一个器官.随着机体的衰老,垂体功能退化是必然趋势.D-半乳糖致衰老模型已被广泛用于研究衰老机制和筛选药物靶标,然而其分子机制尚未清楚.本研究采用差异蛋白质组学方法,以寻找D-半乳糖致衰老小鼠和幼龄小鼠垂体的差异蛋白质,为弄清其功能障碍的分子机制提供新的研究方法和线索.基于双相电泳和质谱结合的方法,本研究发现了46个大于2倍的差异蛋白质,其中32个得到可靠的鉴定(P<0.05).对差异蛋白质功能分析发现,这些显著差异蛋白质主要分布于糖代谢通路,可能与线粒体功能紊乱有关.我们的研究数据为更好地理解D-半乳糖致衰老小鼠垂体功能障碍的内在机制提供了线索.Pituitary gland is critical for the control growth and development in animals.D-galactose induced aging model has been widely used to study the aging mechanism and the screening of drugs.Herein,two dimensional gel electrophoresis（2-DE） followed by mass spectrometry（MS） was performed to exemine proteome differences in pituitary gland between control and D-galactose treated mice.Thereafter,pituitary glands were isolated from controlled and D-galactose treated mice,and pituitary gland proteins were subjected to 2-DE based comparative proteomic analysis（n= 8,4 gels in each group）,which revealed 46 differentially expressed proteins（2 fold）.Among them,32 were identified successfully by mass spectrometry.The results implicated that the altered proteins were mainly involved in glucose metabolism pathway and might contribute to mitochondrial dysfunction.Our data may help to better understanding of molecular mechanisms of pituitary dysfunction in aging mice induced by D-galactose.

关 键 词：垂体 D-半乳糖 衰老 糖代谢 蛋白质组学 质谱 

分 类 号：Q5[生物学—生物化学]