热激蛋白gp96免疫学功能及在主动免疫治疗肿瘤和传染病中的应用  

Immune activity of heat shock protein gp96 and its application in active immunotherapy for tumor and infectious diseases

作　　者：徐亚星[1] 王赛锋[1] 张小俊[1] 孟颂东[1] 

机构地区：[1]中国科学院微生物研究所中国科学院病原微生物与免疫学重点实验室,北京100101

出　　处：《生物工程学报》2012年第3期261-266,共6页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(Nos.30970146;91029724;81021003)资助~~

摘　　要：热激蛋白gp96可特异性结合来源于肿瘤和病毒的抗原肽,与抗原呈递细胞表面CD91等受体作用进入胞内,并在内质网中将结合的抗原通过抗原呈递链呈递给MHCI类分子,激活特异性T细胞。同时,与细胞表面Toll样受体(TLR)TLR2、TLR4等相互作用,激活天然免疫。近期研究发现调节性T细胞(Treg)对gp96免疫功能有显著抑制作用,随着对影响gp96免疫功能的免疫抑制机制的深入了解,以及利用汉逊酵母表达有免疫活性的全长gp96蛋白体系的建立,gp96将在治疗肿瘤及传染性疾病中发挥更大的作用。Heat-shock protein gp96 associates with antigenic peptides derived from tumor and virus.Exogenous gp96-peptide complexes are taken up by antigen-presenting cells through interaction with its receptor CD91 on the cell surface,and cross-present antigenic peptides to MHC class I molecules by a peptide relay line in the endoplasmic reticulum for specific T-cell activation.Meanwhile,gp96 has been shown to initiate innate immune responses through interaction with toll-like receptor 2 and toll-like receptor 4.Recent studies have shown a gp96-mediated immune balance between CTL and Tregs.With the further understanding of counteracting immunosuppressive mechanisms in gp96-induced cellular immune responses,and establishment of high level production of recombinant gp96 by the yeast,gp96 appears to be a promising candidate for designing effective therapeutic vaccines against tumor and infectious diseases.

关 键 词：GP96 T细胞 调节性T细胞 肿瘤 传染病 

分 类 号：R392[医药卫生—免疫学]