正交试验法优选山绿茶中乌苏酸提取工艺  

Optimization of the Extraction Technology of Ursolic Acid from Leaves of Ilex hainanensis by Orthogonal Test

作　　者：程齐来[1] 李洪亮[1] 赵琛 

机构地区：[1]赣南医学院药学院,江西省赣州市341000 [2]江西省赣州市市立医院药剂科,江西省赣州市341000

出　　处：《光谱实验室》2012年第2期1013-1016,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：江西省自然科学基金项目(No.2010GQY0010);江西省教育厅科技计划项目(No.GJJ11206)

摘　　要：筛选山绿茶中乌苏酸的最佳提取工艺。采用正交设计L9(34)考察液固比、提取时间、乙醇浓度和提取次数4个因素,用高效液相色谱法测定山绿茶中乌苏酸的含量。山绿茶中乌苏酸的最佳提取工艺为A2B3C3D1,即选择液固比(mL/g)10∶1、提取时间4h、乙醇浓度90%、提取次数1次。本法工艺合理,质量稳定,适宜于工业化生产。The optimization conditions of extraction technique of ursolic acid（UA） from leaves of Ilex hainanensis was screened.Four factors including liquid-solid ratio,extraction time,ethanol concentration and extraction times were investigated by orthogonal design L9（34）and the content of UA was determined by HPLC.The optimum conditions of extraction technique were A2B3C3D1 as follows：liquid-solid ratio of 10∶1mL/g and extracting only one time（4h for each time） with 90% ethanol.The technique is reasonable with stable quality,and can be used for industrialized production.

关 键 词：山绿茶 乌苏酸 提取工艺 正交试验设计 

分 类 号：O657.72[理学—分析化学]