差示分光光度法测定排石颗粒中的总黄酮  

Determination of Total Flavonoids in Paishi Particles by Differential Spectrophotometry

作　　者：郭爱玲[1] 张淑蓉[1] 刘晓芳[1] 张丽丽[1] 焦建华[1] 裴晓丽[1] 

机构地区：[1]山西中医学院中药系,太原市030024

出　　处：《光谱实验室》2012年第2期1168-1170,共3页Chinese Journal of Spectroscopy Laboratory

基　　金：山西中医学院"十一五"科技产业规划项目(2006);山西省科技厅科技攻关项目(2006031083-01)

摘　　要：以芦丁为观测指标,在505nm波长下,差示分光光度法测定排石颗粒中总黄酮含量。芦丁对照品在15.48—54.18μg/mL具有良好线性关系,平均加样回收率为98.05%。该方法操作简便、结果准确、重现性好,可作为排石颗粒生产质量控制检测方法。The contents of total flaconids in the paishi particles were determined by differential spetrophotometry with lutin as observation index at wavelength of 505nm.There was a good linear relationship for the concentration of lutin reference substance in the range of 15.48—54.18μg/mL.The average recovery was 98.05%.The method is simple,accurate with good repeatability,and that can be applied to determine the quality control of paishi particles.

关 键 词：排石颗粒 总黄酮 差示分光光度法 

分 类 号：O657.32[理学—分析化学]