膜荚黄芪子叶节植株再生体系的建立  

Establishment of Plantlet Regeneration System of Cotyledon Node for Astragalus membranaceus

作　　者：王树斌[1] 全雪丽[1] 周玉红[1] 吴松权[1] 

机构地区：[1]延边大学农学院,吉林延吉133002

出　　处：《北方园艺》2012年第6期177-178,共2页Northern Horticulture

基　　金：国家自然科学基金资助项目(30860036);吉林省自然科学基金资助项目(201115228)

摘　　要：以膜荚黄芪无菌苗子叶节为外植体,建立了简单、高效和稳定的膜荚黄芪再生体系。结果表明:不定芽诱导的最适培养基是MS+6-BA 1.0mg/L+NAA 0.10mg/L,每个子叶节外植体平均出芽数为3个以上,再生频率达75.5%;生根最适培养基为1/2MS+IBA 0.5mg/L;移栽后成活率达75%以上。The cotyledon node obtained from aseptic seedling of Astragalus mend）ranaceus were used as the explant and a simple,stable and high efficient regeneration system for A. membranaceus were established. The results showed that the optimum medium for adventitious shoot regeneration was MS＋6-BA 1.0 mg/L＋NAA 0. 10 mg/L,the average number of differential buds per cotyledon node was above 3,and the regenerated frequency was 75.5% . The suitable rooting medium was 1/2MS＋IBA 0. 5 mg/L and the survive rate was above 75% after transplantation.

关 键 词：膜荚黄芪 子叶节 再生体系 

分 类 号：S567.53[农业科学—中草药栽培]